Customized one-step preparation of sgRNA transcription templates via overlapping PCR Using short primers and its application in vitro and in vivo gene editing

Overlap extension polymerase chain reaction (PCR) is a powerful technology for DNA assembly. Based on this technology, we synthesized DNA templates, which were transcribed into sgRNA in vitro, and further detected their efficiency of purified sgRNAs with Cas9 nuclease. The sgRNAs synthesized by this...

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Detalles Bibliográficos
Autores principales: Hu, Zheng, Wang, Li, Shi, Zhaoying, Jiang, Jing, Li, Xiangning, Chen, Yonglong, Li, Kai, Luo, Dixian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Letter to the Editor
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6814055/
https://www.ncbi.nlm.nih.gov/pubmed/31673328
http://dx.doi.org/10.1186/s13578-019-0350-7

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6814055/
https://www.ncbi.nlm.nih.gov/pubmed/31673328
http://dx.doi.org/10.1186/s13578-019-0350-7

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