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Slow freezing versus vitrification for the cryopreservation of zebrafish (Danio rerio) ovarian tissue

The aim of the present study was to compare the efficiency of vitrification and slow freezing techniques for the cryopreservation of zebrafish ovarian tissue containing immature follicles. In Experiment 1, assessment of cell membrane integrity by trypan blue exclusion staining was used to select the...

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Detalles Bibliográficos
Autores principales: Marques, Lis S., Fossati, Ana A. N., Rodrigues, Rômulo B., Da Rosa, Helen T., Izaguirry, Aryele P., Ramalho, Juliana B., Moreira, José C. F., Santos, Francielli Weber, Zhang, Tiantian, Streit, Danilo P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6814760/
https://www.ncbi.nlm.nih.gov/pubmed/31653925
http://dx.doi.org/10.1038/s41598-019-51696-7