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Slow freezing versus vitrification for the cryopreservation of zebrafish (Danio rerio) ovarian tissue
The aim of the present study was to compare the efficiency of vitrification and slow freezing techniques for the cryopreservation of zebrafish ovarian tissue containing immature follicles. In Experiment 1, assessment of cell membrane integrity by trypan blue exclusion staining was used to select the...
Autores principales: | Marques, Lis S., Fossati, Ana A. N., Rodrigues, Rômulo B., Da Rosa, Helen T., Izaguirry, Aryele P., Ramalho, Juliana B., Moreira, José C. F., Santos, Francielli Weber, Zhang, Tiantian, Streit, Danilo P. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6814760/ https://www.ncbi.nlm.nih.gov/pubmed/31653925 http://dx.doi.org/10.1038/s41598-019-51696-7 |
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