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Invariant NKT cells facilitate cytotoxic T-cell activation via direct recognition of CD1d on T cells
Invariant natural killer T (iNKT) cells are a major subset of NKT cells that recognize foreign and endogenous lipid antigens presented by CD1d. Although iNKT cells are characteristically autoreactive to self-antigens, the role of iNKT cells in the regulation of cytotoxic T lymphocytes (CTL) has been...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6814837/ https://www.ncbi.nlm.nih.gov/pubmed/31653827 http://dx.doi.org/10.1038/s12276-019-0329-9 |
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author | Qin, Yingyu Oh, Sejin Lim, Sojung Shin, Jung Hoon Yoon, Min Sang Park, Se-Ho |
author_facet | Qin, Yingyu Oh, Sejin Lim, Sojung Shin, Jung Hoon Yoon, Min Sang Park, Se-Ho |
author_sort | Qin, Yingyu |
collection | PubMed |
description | Invariant natural killer T (iNKT) cells are a major subset of NKT cells that recognize foreign and endogenous lipid antigens presented by CD1d. Although iNKT cells are characteristically autoreactive to self-antigens, the role of iNKT cells in the regulation of cytotoxic T lymphocytes (CTL) has been elucidated using α-galactosylceramide (α-GalCer), a strong synthetic glycolipid that is presented by professional antigen presenting cells (APCs), such as dendritic cells. Despite the well-known effects of α-GalCer and dendritic cells on lipid antigen presentation, the physiological role of endogenous antigens presented by CTLs during crosstalk with iNKT cells has not yet been addressed. In this study, we found that antigen-primed CTLs with transient CD1d upregulation could present lipid self-antigens to activate the iNKT cell production of IFN-γ. CTL-mediated iNKT cell activation in turn enhanced IFN-γ production and the proliferation and cytotoxicity of CTLs. We also found that the direct interaction of iNKT cells and CTLs enhanced the antitumor immune responses of CTLs. This partially explains the functional role of iNKT cells in CTL-mediated antitumor immunity. Our findings suggest that in the absence of exogenous iNKT cell ligands, iNKT cells enhanced the CTL production of IFN-γ and CTL proliferation and cytotoxicity via direct interaction with CD1d expressed on T cells without interacting with APCs. |
format | Online Article Text |
id | pubmed-6814837 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-68148372019-10-30 Invariant NKT cells facilitate cytotoxic T-cell activation via direct recognition of CD1d on T cells Qin, Yingyu Oh, Sejin Lim, Sojung Shin, Jung Hoon Yoon, Min Sang Park, Se-Ho Exp Mol Med Article Invariant natural killer T (iNKT) cells are a major subset of NKT cells that recognize foreign and endogenous lipid antigens presented by CD1d. Although iNKT cells are characteristically autoreactive to self-antigens, the role of iNKT cells in the regulation of cytotoxic T lymphocytes (CTL) has been elucidated using α-galactosylceramide (α-GalCer), a strong synthetic glycolipid that is presented by professional antigen presenting cells (APCs), such as dendritic cells. Despite the well-known effects of α-GalCer and dendritic cells on lipid antigen presentation, the physiological role of endogenous antigens presented by CTLs during crosstalk with iNKT cells has not yet been addressed. In this study, we found that antigen-primed CTLs with transient CD1d upregulation could present lipid self-antigens to activate the iNKT cell production of IFN-γ. CTL-mediated iNKT cell activation in turn enhanced IFN-γ production and the proliferation and cytotoxicity of CTLs. We also found that the direct interaction of iNKT cells and CTLs enhanced the antitumor immune responses of CTLs. This partially explains the functional role of iNKT cells in CTL-mediated antitumor immunity. Our findings suggest that in the absence of exogenous iNKT cell ligands, iNKT cells enhanced the CTL production of IFN-γ and CTL proliferation and cytotoxicity via direct interaction with CD1d expressed on T cells without interacting with APCs. Nature Publishing Group UK 2019-10-25 /pmc/articles/PMC6814837/ /pubmed/31653827 http://dx.doi.org/10.1038/s12276-019-0329-9 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Qin, Yingyu Oh, Sejin Lim, Sojung Shin, Jung Hoon Yoon, Min Sang Park, Se-Ho Invariant NKT cells facilitate cytotoxic T-cell activation via direct recognition of CD1d on T cells |
title | Invariant NKT cells facilitate cytotoxic T-cell activation via direct recognition of CD1d on T cells |
title_full | Invariant NKT cells facilitate cytotoxic T-cell activation via direct recognition of CD1d on T cells |
title_fullStr | Invariant NKT cells facilitate cytotoxic T-cell activation via direct recognition of CD1d on T cells |
title_full_unstemmed | Invariant NKT cells facilitate cytotoxic T-cell activation via direct recognition of CD1d on T cells |
title_short | Invariant NKT cells facilitate cytotoxic T-cell activation via direct recognition of CD1d on T cells |
title_sort | invariant nkt cells facilitate cytotoxic t-cell activation via direct recognition of cd1d on t cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6814837/ https://www.ncbi.nlm.nih.gov/pubmed/31653827 http://dx.doi.org/10.1038/s12276-019-0329-9 |
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