Isolation of secreted proteins from Drosophila ovaries and embryos through in vivo BirA-mediated biotinylation

The extraordinarily strong non-covalent interaction between biotin and avidin (kD = 10(−14)–10(−16)) has permitted this interaction to be used in a wide variety of experimental contexts. The Biotin Acceptor Peptide (BAP), a 15 amino acid motif that can be biotinylated by the E. coli BirA protein, ha...

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Detalles Bibliográficos
Autores principales: Stevens, Leslie M., Zhang, Yuan, Volnov, Yuri, Chen, Geng, Stein, David S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6816556/
https://www.ncbi.nlm.nih.gov/pubmed/31658274
http://dx.doi.org/10.1371/journal.pone.0219878

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6816556/
https://www.ncbi.nlm.nih.gov/pubmed/31658274
http://dx.doi.org/10.1371/journal.pone.0219878

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