Cargando…
Isolation of secreted proteins from Drosophila ovaries and embryos through in vivo BirA-mediated biotinylation
The extraordinarily strong non-covalent interaction between biotin and avidin (kD = 10(−14)–10(−16)) has permitted this interaction to be used in a wide variety of experimental contexts. The Biotin Acceptor Peptide (BAP), a 15 amino acid motif that can be biotinylated by the E. coli BirA protein, ha...
| Autores principales: | , , , , |
|---|---|
| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Public Library of Science
2019
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6816556/ https://www.ncbi.nlm.nih.gov/pubmed/31658274 http://dx.doi.org/10.1371/journal.pone.0219878 |