Preparation of a new type 2 diabetic miniature pig model via the CRISPR/Cas9 system

Diabetes has become one of the major noninfectious diseases that seriously endanger public health. The formation of islet amyloid polypeptide (IAPP) affects the normal physiological functions of the body, such as glucose metabolism and lipid metabolism. The mature human IAPP protein (hIAPP) has a st...

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Autores principales: Zou, Xiaodong, Ouyang, Hongsheng, Yu, Tingting, Chen, Xue, Pang, Daxin, Tang, Xiaochun, Chen, Chengzhen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6817862/
https://www.ncbi.nlm.nih.gov/pubmed/31659151
http://dx.doi.org/10.1038/s41419-019-2056-5
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author Zou, Xiaodong
Ouyang, Hongsheng
Yu, Tingting
Chen, Xue
Pang, Daxin
Tang, Xiaochun
Chen, Chengzhen
author_facet Zou, Xiaodong
Ouyang, Hongsheng
Yu, Tingting
Chen, Xue
Pang, Daxin
Tang, Xiaochun
Chen, Chengzhen
author_sort Zou, Xiaodong
collection PubMed
description Diabetes has become one of the major noninfectious diseases that seriously endanger public health. The formation of islet amyloid polypeptide (IAPP) affects the normal physiological functions of the body, such as glucose metabolism and lipid metabolism. The mature human IAPP protein (hIAPP) has a strong tendency to misfold and is considered to be one of the major causes of amyloid changes in islets. Deposition of hIAPP is considered to be one of the leading causes of type 2 diabetes mellitus (T2DM). Miniature pigs are experimental animal models that are well suited for research on gene function and human diabetes. In our study, we obtained IAPP gene-humanized miniature pigs via the CRISPR/Cas9 system and somatic cell nuclear transfer (SCNT) technology. The hIAPP pigs can be used to further study the pathogenesis and related complications of T2DM and to lay a solid foundation for the prevention and treatment of T2DM.
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spelling pubmed-68178622019-10-29 Preparation of a new type 2 diabetic miniature pig model via the CRISPR/Cas9 system Zou, Xiaodong Ouyang, Hongsheng Yu, Tingting Chen, Xue Pang, Daxin Tang, Xiaochun Chen, Chengzhen Cell Death Dis Article Diabetes has become one of the major noninfectious diseases that seriously endanger public health. The formation of islet amyloid polypeptide (IAPP) affects the normal physiological functions of the body, such as glucose metabolism and lipid metabolism. The mature human IAPP protein (hIAPP) has a strong tendency to misfold and is considered to be one of the major causes of amyloid changes in islets. Deposition of hIAPP is considered to be one of the leading causes of type 2 diabetes mellitus (T2DM). Miniature pigs are experimental animal models that are well suited for research on gene function and human diabetes. In our study, we obtained IAPP gene-humanized miniature pigs via the CRISPR/Cas9 system and somatic cell nuclear transfer (SCNT) technology. The hIAPP pigs can be used to further study the pathogenesis and related complications of T2DM and to lay a solid foundation for the prevention and treatment of T2DM. Nature Publishing Group UK 2019-10-28 /pmc/articles/PMC6817862/ /pubmed/31659151 http://dx.doi.org/10.1038/s41419-019-2056-5 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Zou, Xiaodong
Ouyang, Hongsheng
Yu, Tingting
Chen, Xue
Pang, Daxin
Tang, Xiaochun
Chen, Chengzhen
Preparation of a new type 2 diabetic miniature pig model via the CRISPR/Cas9 system
title Preparation of a new type 2 diabetic miniature pig model via the CRISPR/Cas9 system
title_full Preparation of a new type 2 diabetic miniature pig model via the CRISPR/Cas9 system
title_fullStr Preparation of a new type 2 diabetic miniature pig model via the CRISPR/Cas9 system
title_full_unstemmed Preparation of a new type 2 diabetic miniature pig model via the CRISPR/Cas9 system
title_short Preparation of a new type 2 diabetic miniature pig model via the CRISPR/Cas9 system
title_sort preparation of a new type 2 diabetic miniature pig model via the crispr/cas9 system
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6817862/
https://www.ncbi.nlm.nih.gov/pubmed/31659151
http://dx.doi.org/10.1038/s41419-019-2056-5
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