Multiplex PCR-based titration (MPBT) assay for determination of infectious titers of the three Sabin strains of live poliovirus vaccine

BACKGROUND: Conventional assays to titrate polioviruses usually test serial dilutions inoculated into replicate cell cultures to determine a 50% cytopathic endpoint, a process that is both time-consuming and laborious. Such a method is still used to measure potency of live Oral Poliovirus Vaccine du...

Descripción completa

Detalles Bibliográficos
Autores principales: Manukyan, Hasmik, Rodionova, Elvira, Zagorodnyaya, Tatiana, Lin, Tsai-Lien, Chumakov, Konstantin, Laassri, Majid
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Methodology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6819588/
https://www.ncbi.nlm.nih.gov/pubmed/31660997
http://dx.doi.org/10.1186/s12985-019-1233-6

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6819588/
https://www.ncbi.nlm.nih.gov/pubmed/31660997
http://dx.doi.org/10.1186/s12985-019-1233-6

Ejemplares similares