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Multiplex PCR-based titration (MPBT) assay for determination of infectious titers of the three Sabin strains of live poliovirus vaccine

BACKGROUND: Conventional assays to titrate polioviruses usually test serial dilutions inoculated into replicate cell cultures to determine a 50% cytopathic endpoint, a process that is both time-consuming and laborious. Such a method is still used to measure potency of live Oral Poliovirus Vaccine du...

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Detalles Bibliográficos
Autores principales:	Manukyan, Hasmik, Rodionova, Elvira, Zagorodnyaya, Tatiana, Lin, Tsai-Lien, Chumakov, Konstantin, Laassri, Majid
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2019
Materias:	Methodology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6819588/ https://www.ncbi.nlm.nih.gov/pubmed/31660997 http://dx.doi.org/10.1186/s12985-019-1233-6

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