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Enzyme-free release of adhered cells from standard culture dishes using intermittent ultrasonic traveling waves

Cell detachment is essential in culturing adherent cells. Trypsinization is the most popular detachment technique, even though it reduces viability due to the damage to the membrane and extracellular matrix. Avoiding such damage would improve cell culture efficiency. Here we propose an enzyme-free c...

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Detalles Bibliográficos
Autores principales: Kurashina, Yuta, Imashiro, Chikahiro, Hirano, Makoto, Kuribara, Taiki, Totani, Kiichiro, Ohnuma, Kiyoshi, Friend, James, Takemura, Kenjiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6820801/
https://www.ncbi.nlm.nih.gov/pubmed/31701022
http://dx.doi.org/10.1038/s42003-019-0638-5