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Enzyme-free release of adhered cells from standard culture dishes using intermittent ultrasonic traveling waves
Cell detachment is essential in culturing adherent cells. Trypsinization is the most popular detachment technique, even though it reduces viability due to the damage to the membrane and extracellular matrix. Avoiding such damage would improve cell culture efficiency. Here we propose an enzyme-free c...
Autores principales: | Kurashina, Yuta, Imashiro, Chikahiro, Hirano, Makoto, Kuribara, Taiki, Totani, Kiichiro, Ohnuma, Kiyoshi, Friend, James, Takemura, Kenjiro |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6820801/ https://www.ncbi.nlm.nih.gov/pubmed/31701022 http://dx.doi.org/10.1038/s42003-019-0638-5 |
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