An improved overlap extension PCR for simultaneous multiple sites large fragments insertion, deletion and substitution

The existing molecular cloning methods are often limited by the availability of suitable restriction sites. It is still a challenge for simultaneous cloning of multiple fragments into different sites of a single vector. Here we developed a novel method named improved overlap extension PCR (IOEP) for...

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Detalles Bibliográficos
Autores principales: Guo, Wei, Xie, Binhua, Jiang, Min, Zhu, Xiao-Jing, Qiu, Mengsheng, Dai, Zhong-Min
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6821815/
https://www.ncbi.nlm.nih.gov/pubmed/31666610
http://dx.doi.org/10.1038/s41598-019-52122-8

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6821815/
https://www.ncbi.nlm.nih.gov/pubmed/31666610
http://dx.doi.org/10.1038/s41598-019-52122-8

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