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An improved overlap extension PCR for simultaneous multiple sites large fragments insertion, deletion and substitution
The existing molecular cloning methods are often limited by the availability of suitable restriction sites. It is still a challenge for simultaneous cloning of multiple fragments into different sites of a single vector. Here we developed a novel method named improved overlap extension PCR (IOEP) for...
| Autores principales: | , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group UK
2019
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6821815/ https://www.ncbi.nlm.nih.gov/pubmed/31666610 http://dx.doi.org/10.1038/s41598-019-52122-8 |