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DNA amplification with in situ nucleoside to dNTP synthesis, using a single recombinant cell lysate of E. coli
Nucleic acid amplification (NAA) is a cornerstone of modern molecular and synthetic biology. Routine application by non-specialists, however, is hampered by difficulties with storing and handling the requisite labile and expensive reagents, such as deoxynucleoside triphosphates (dNTPs) and polymeras...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Nature Publishing Group UK
2019
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6821818/ https://www.ncbi.nlm.nih.gov/pubmed/31666578 http://dx.doi.org/10.1038/s41598-019-51917-z |