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Native adiponectin in serum binds to mammalian cells expressing T-cadherin, but not AdipoRs or calreticulin

Adiponectin is an adipocyte-derived atypically abundant circulating factor that protects various organs and tissues through its receptors, AdipoRs, calreticulin, and T-cadherin. To identify the major binding partner of circulating native adiponectin, we expressed these receptors on the surface of HE...

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Autores principales: Kita, Shunbun, Fukuda, Shiro, Maeda, Norikazu, Shimomura, Iichiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6822988/
https://www.ncbi.nlm.nih.gov/pubmed/31647413
http://dx.doi.org/10.7554/eLife.48675
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author Kita, Shunbun
Fukuda, Shiro
Maeda, Norikazu
Shimomura, Iichiro
author_facet Kita, Shunbun
Fukuda, Shiro
Maeda, Norikazu
Shimomura, Iichiro
author_sort Kita, Shunbun
collection PubMed
description Adiponectin is an adipocyte-derived atypically abundant circulating factor that protects various organs and tissues through its receptors, AdipoRs, calreticulin, and T-cadherin. To identify the major binding partner of circulating native adiponectin, we expressed these receptors on the surface of HEK293 cells. Adiponectin, either that in mouse or human serum, purified from serum, or produced by mammalian cells, bound to cells expressing T-cadherin, but not to those expressing AdipoR1 or calreticulin. The stable introduction of T-cadherin and AdipoR1 into CHO cells resulted in the cell surface localization of these receptors. Native adiponectin in serum bound to cells expressing T-cadherin, not to those expressing AdipoR1. The knockdown of T-cadherin, but not AdipoRs resulted in the significant attenuation of native adiponectin binding to C2C12 myotubes. Therefore, native adiponectin binding depended on the amount of T-cadherin expressed in HEK293 cells, CHO cells, and C2C12 myotubes. Collectively, our mammalian cell-based studies suggest that T-cadherin is the major binding partner of native adiponectin in serum.
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spelling pubmed-68229882019-11-04 Native adiponectin in serum binds to mammalian cells expressing T-cadherin, but not AdipoRs or calreticulin Kita, Shunbun Fukuda, Shiro Maeda, Norikazu Shimomura, Iichiro eLife Cell Biology Adiponectin is an adipocyte-derived atypically abundant circulating factor that protects various organs and tissues through its receptors, AdipoRs, calreticulin, and T-cadherin. To identify the major binding partner of circulating native adiponectin, we expressed these receptors on the surface of HEK293 cells. Adiponectin, either that in mouse or human serum, purified from serum, or produced by mammalian cells, bound to cells expressing T-cadherin, but not to those expressing AdipoR1 or calreticulin. The stable introduction of T-cadherin and AdipoR1 into CHO cells resulted in the cell surface localization of these receptors. Native adiponectin in serum bound to cells expressing T-cadherin, not to those expressing AdipoR1. The knockdown of T-cadherin, but not AdipoRs resulted in the significant attenuation of native adiponectin binding to C2C12 myotubes. Therefore, native adiponectin binding depended on the amount of T-cadherin expressed in HEK293 cells, CHO cells, and C2C12 myotubes. Collectively, our mammalian cell-based studies suggest that T-cadherin is the major binding partner of native adiponectin in serum. eLife Sciences Publications, Ltd 2019-10-24 /pmc/articles/PMC6822988/ /pubmed/31647413 http://dx.doi.org/10.7554/eLife.48675 Text en © 2019, Kita et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Cell Biology
Kita, Shunbun
Fukuda, Shiro
Maeda, Norikazu
Shimomura, Iichiro
Native adiponectin in serum binds to mammalian cells expressing T-cadherin, but not AdipoRs or calreticulin
title Native adiponectin in serum binds to mammalian cells expressing T-cadherin, but not AdipoRs or calreticulin
title_full Native adiponectin in serum binds to mammalian cells expressing T-cadherin, but not AdipoRs or calreticulin
title_fullStr Native adiponectin in serum binds to mammalian cells expressing T-cadherin, but not AdipoRs or calreticulin
title_full_unstemmed Native adiponectin in serum binds to mammalian cells expressing T-cadherin, but not AdipoRs or calreticulin
title_short Native adiponectin in serum binds to mammalian cells expressing T-cadherin, but not AdipoRs or calreticulin
title_sort native adiponectin in serum binds to mammalian cells expressing t-cadherin, but not adipors or calreticulin
topic Cell Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6822988/
https://www.ncbi.nlm.nih.gov/pubmed/31647413
http://dx.doi.org/10.7554/eLife.48675
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