Measuring Rapid A–Ci Curves in Boreal Conifers: Black Spruce and Balsam Fir

Climate change is steering tree breeding programs towards the development of families and genotypes that will be adapted and more resilient to changing environments. Making genotype–phenotype–environment connections is central to these predictions and it requires the evaluation of functional traits...

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Autores principales: Coursolle, Carole, Otis Prud’homme, Guillaume, Lamothe, Manuel, Isabel, Nathalie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6823239/
https://www.ncbi.nlm.nih.gov/pubmed/31708940
http://dx.doi.org/10.3389/fpls.2019.01276
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author Coursolle, Carole
Otis Prud’homme, Guillaume
Lamothe, Manuel
Isabel, Nathalie
author_facet Coursolle, Carole
Otis Prud’homme, Guillaume
Lamothe, Manuel
Isabel, Nathalie
author_sort Coursolle, Carole
collection PubMed
description Climate change is steering tree breeding programs towards the development of families and genotypes that will be adapted and more resilient to changing environments. Making genotype–phenotype–environment connections is central to these predictions and it requires the evaluation of functional traits such as photosynthetic rates that can be linked to environmental variables. However, the ability to rapidly measure photosynthetic parameters has always been limiting. The estimation of V (c,max) and J (max) using CO(2) response curves has traditionally been time consuming, taking anywhere from 30 min to more than an hour, thereby drastically limiting the number of trees that can be assessed per day. Technological advancements have led to the development of a new generation of portable photosynthesis measurement systems offering greater chamber environmental control and automated sampling and, as a result, the proposal of a new, faster, method (RACiR) for measuring V (c,max)and J (max). This method was developed using poplar trees and involves measuring photosynthetic responses to CO(2) over a range of CO(2) concentrations changing at a constant rate. The goal of the present study was to adapt the RACiR method for use on conifers whose measurement usually requires much larger leaf chambers. We demonstrate that the RACiR method can be used to estimate V (c,max) and J (max) in conifers and provide recommendations to enhance the method. The use our method in conifers will substantially reduce measurement time, thus greatly improving genotype evaluation and selection capabilities based on photosynthetic traits. This study led to the developpement of an R package (RapidACi, https://github.com/ManuelLamothe/RapidACi) that facilitates the correction of multiple RACiR files and the post-measurement correction of leaf areas.
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spelling pubmed-68232392019-11-08 Measuring Rapid A–Ci Curves in Boreal Conifers: Black Spruce and Balsam Fir Coursolle, Carole Otis Prud’homme, Guillaume Lamothe, Manuel Isabel, Nathalie Front Plant Sci Plant Science Climate change is steering tree breeding programs towards the development of families and genotypes that will be adapted and more resilient to changing environments. Making genotype–phenotype–environment connections is central to these predictions and it requires the evaluation of functional traits such as photosynthetic rates that can be linked to environmental variables. However, the ability to rapidly measure photosynthetic parameters has always been limiting. The estimation of V (c,max) and J (max) using CO(2) response curves has traditionally been time consuming, taking anywhere from 30 min to more than an hour, thereby drastically limiting the number of trees that can be assessed per day. Technological advancements have led to the development of a new generation of portable photosynthesis measurement systems offering greater chamber environmental control and automated sampling and, as a result, the proposal of a new, faster, method (RACiR) for measuring V (c,max)and J (max). This method was developed using poplar trees and involves measuring photosynthetic responses to CO(2) over a range of CO(2) concentrations changing at a constant rate. The goal of the present study was to adapt the RACiR method for use on conifers whose measurement usually requires much larger leaf chambers. We demonstrate that the RACiR method can be used to estimate V (c,max) and J (max) in conifers and provide recommendations to enhance the method. The use our method in conifers will substantially reduce measurement time, thus greatly improving genotype evaluation and selection capabilities based on photosynthetic traits. This study led to the developpement of an R package (RapidACi, https://github.com/ManuelLamothe/RapidACi) that facilitates the correction of multiple RACiR files and the post-measurement correction of leaf areas. Frontiers Media S.A. 2019-10-25 /pmc/articles/PMC6823239/ /pubmed/31708940 http://dx.doi.org/10.3389/fpls.2019.01276 Text en Copyright © 2019 Her Majesty the Queen in Right of Canada http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Coursolle, Carole
Otis Prud’homme, Guillaume
Lamothe, Manuel
Isabel, Nathalie
Measuring Rapid A–Ci Curves in Boreal Conifers: Black Spruce and Balsam Fir
title Measuring Rapid A–Ci Curves in Boreal Conifers: Black Spruce and Balsam Fir
title_full Measuring Rapid A–Ci Curves in Boreal Conifers: Black Spruce and Balsam Fir
title_fullStr Measuring Rapid A–Ci Curves in Boreal Conifers: Black Spruce and Balsam Fir
title_full_unstemmed Measuring Rapid A–Ci Curves in Boreal Conifers: Black Spruce and Balsam Fir
title_short Measuring Rapid A–Ci Curves in Boreal Conifers: Black Spruce and Balsam Fir
title_sort measuring rapid a–ci curves in boreal conifers: black spruce and balsam fir
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6823239/
https://www.ncbi.nlm.nih.gov/pubmed/31708940
http://dx.doi.org/10.3389/fpls.2019.01276
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