Split intein-mediated selection of cells containing two plasmids using a single antibiotic

To build or dissect complex pathways in bacteria and mammalian cells, it is often necessary to recur to at least two plasmids, for instance harboring orthogonal inducible promoters. Here we present SiMPl, a method based on rationally designed split enzymes and intein-mediated protein trans-splicing,...

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Detalles Bibliográficos
Autores principales: Palanisamy, Navaneethan, Degen, Anna, Morath, Anna, Ballestin Ballestin, Jara, Juraske, Claudia, Öztürk, Mehmet Ali, Sprenger, Georg A., Youn, Jung-Won, Schamel, Wolfgang W., Di Ventura, Barbara
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6823396/
https://www.ncbi.nlm.nih.gov/pubmed/31672972
http://dx.doi.org/10.1038/s41467-019-12911-1

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6823396/
https://www.ncbi.nlm.nih.gov/pubmed/31672972
http://dx.doi.org/10.1038/s41467-019-12911-1

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