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Split intein-mediated selection of cells containing two plasmids using a single antibiotic
To build or dissect complex pathways in bacteria and mammalian cells, it is often necessary to recur to at least two plasmids, for instance harboring orthogonal inducible promoters. Here we present SiMPl, a method based on rationally designed split enzymes and intein-mediated protein trans-splicing,...
Autores principales: | Palanisamy, Navaneethan, Degen, Anna, Morath, Anna, Ballestin Ballestin, Jara, Juraske, Claudia, Öztürk, Mehmet Ali, Sprenger, Georg A., Youn, Jung-Won, Schamel, Wolfgang W., Di Ventura, Barbara |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6823396/ https://www.ncbi.nlm.nih.gov/pubmed/31672972 http://dx.doi.org/10.1038/s41467-019-12911-1 |
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