Specific growth rate governs AOX1 gene expression, affecting the production kinetics of Pichia pastoris (Komagataella phaffii) P(AOX1)-driven recombinant producer strains with different target gene dosage

BACKGROUND: The P(AOX1)-based expression system is the most widely used for producing recombinant proteins in the methylotrophic yeast Pichia pastoris (Komagataella phaffii). Despite relevant recent advances in regulation of the methanol utilization (MUT) pathway have been made, the role of specific...

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Detalles Bibliográficos
Autores principales: Garrigós-Martínez, Javier, Nieto-Taype, Miguel Angel, Gasset-Franch, Arnau, Montesinos-Seguí, José Luis, Garcia-Ortega, Xavier, Valero, Francisco
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6824138/
https://www.ncbi.nlm.nih.gov/pubmed/31675969
http://dx.doi.org/10.1186/s12934-019-1240-8

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6824138/
https://www.ncbi.nlm.nih.gov/pubmed/31675969
http://dx.doi.org/10.1186/s12934-019-1240-8

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