Cargando…

Specific growth rate governs AOX1 gene expression, affecting the production kinetics of Pichia pastoris (Komagataella phaffii) P(AOX1)-driven recombinant producer strains with different target gene dosage

BACKGROUND: The P(AOX1)-based expression system is the most widely used for producing recombinant proteins in the methylotrophic yeast Pichia pastoris (Komagataella phaffii). Despite relevant recent advances in regulation of the methanol utilization (MUT) pathway have been made, the role of specific...

Descripción completa

Detalles Bibliográficos
Autores principales:	Garrigós-Martínez, Javier, Nieto-Taype, Miguel Angel, Gasset-Franch, Arnau, Montesinos-Seguí, José Luis, Garcia-Ortega, Xavier, Valero, Francisco
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2019
Materias:	Research
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6824138/ https://www.ncbi.nlm.nih.gov/pubmed/31675969 http://dx.doi.org/10.1186/s12934-019-1240-8

Ejemplares similares

Rationale‐based selection of optimal operating strategies and gene dosage impact on recombinant protein production in Komagataella phaffii (Pichia pastoris)
por: Nieto‐Taype, Miguel Angel, et al.
Publicado: (2019)

P( AOX1 ) expression in mixed-substrate continuous cultures of Komagataella phaffii (Pichia pastoris) is completely determined by methanol consumption regardless of the secondary carbon source
por: Singh, Anamika, et al.
Publicado: (2023)

Industrial Production of Proteins with Pichia pastoris—Komagataella phaffii
por: Barone, Giovanni Davide, et al.
Publicado: (2023)

Evaluation of reference genes for transcript analyses in Komagataella phaffii (Pichia pastoris)
por: Besleaga, Mihail, et al.
Publicado: (2023)

Increased dosage of AOX1 promoter-regulated expression cassettes leads to transcription attenuation of the methanol metabolism in Pichia pastoris
por: Cámara, Elena, et al.
Publicado: (2017)

Controlling AOX1 promoter strength in Pichia pastoris by manipulating poly (dA:dT) tracts
por: Yang, Jun, et al.
Publicado: (2018)

An effective combination of codon optimization, gene dosage, and process optimization for high-level production of fibrinolytic enzyme in Komagataella phaffii (Pichia pastoris)
por: Che, Zhiqun, et al.
Publicado: (2020)

Heterologous Expression and Bioactivity Determination of Monochamus alternatus Antibacterial Peptide Gene in Komagataella phaffii (Pichia pastoris)
por: Chu, Xu, et al.
Publicado: (2023)

Role of Dissimilative Pathway of Komagataella phaffii (Pichia pastoris): Formaldehyde Toxicity and Energy Metabolism
por: Berrios, Julio, et al.
Publicado: (2022)

Exploring the genetic architecture of protein secretion in Komagataella phaffii (Pichia pastoris) for biotechnology applications
por: Swichkow, Chantle Reiko, et al.
Publicado: (2022)

Mit1 Transcription Factor Mediates Methanol Signaling and Regulates the Alcohol Oxidase 1 (AOX1) Promoter in Pichia pastoris
por: Wang, Xiaolong, et al.
Publicado: (2016)

Innovative Bioprocess Strategies Combining Physiological Control and Strain Engineering of Pichia pastoris to Improve Recombinant Protein Production
por: Gasset, Arnau, et al.
Publicado: (2022)

Centromeres of the Yeast Komagataella phaffii (Pichia pastoris) Have a Simple Inverted-Repeat Structure
por: Coughlan, Aisling Y., et al.
Publicado: (2016)

Genomic diversity and meiotic recombination among isolates of the biotech yeast Komagataella phaffii (Pichia pastoris)
por: Braun-Galleani, Stephanie, et al.
Publicado: (2019)

Enhancing the efficiency of the Pichia pastoris AOX1 promoter via the synthetic positive feedback circuit of transcription factor Mxr1
por: Chang, Ching-Hsiang, et al.
Publicado: (2018)

Methanol-Independent Protein Expression by AOX1 Promoter with trans-Acting Elements Engineering and Glucose-Glycerol-Shift Induction in Pichia pastoris
por: Wang, Jinjia, et al.
Publicado: (2017)

Kinase Screening in Pichia pastoris Identified Promising Targets Involved in Cell Growth and Alcohol Oxidase 1 Promoter (P(AOX1)) Regulation
por: Shen, Wei, et al.
Publicado: (2016)

Engineering the expression system for Komagataella phaffii (Pichia pastoris): an attempt to develop a methanol-free expression system
por: Takagi, Shinobu, et al.
Publicado: (2019)

A quantitative study of methanol/sorbitol co-feeding process of a Pichia pastoris Mut(+)/pAOX1-lacZ strain
por: Niu, Hongxing, et al.
Publicado: (2013)

Effect of Pyruvate Decarboxylase Knockout on Product Distribution Using Pichia pastoris (Komagataella phaffii) Engineered for Lactic Acid Production
por: Melo, Nadiele T. M., et al.
Publicado: (2018)

Establishment of a co-culture system using Escherichia coli and Pichia pastoris (Komagataella phaffii) for valuable alkaloid production
por: Urui, Miya, et al.
Publicado: (2021)

Pichia pastoris (Komagataella phaffii) as a Cost-Effective Tool for Vaccine Production for Low- and Middle-Income Countries (LMICs)
por: de Sá Magalhães, Salomé, et al.
Publicado: (2021)

Identification of genetic variants of the industrial yeast Komagataella phaffii (Pichia pastoris) that contribute to increased yields of secreted heterologous proteins
por: Offei, Benjamin, et al.
Publicado: (2022)

Wild Carrot Differentiation in Europe and Selection at DcAOX1 Gene?
por: Nobre, Tânia, et al.
Publicado: (2016)

Continuous Cultivation as a Tool Toward the Rational Bioprocess Development With Pichia Pastoris Cell Factory
por: Nieto-Taype, Miguel Angel, et al.
Publicado: (2020)

Strategies for the production of isotopically labelled Fab fragments of therapeutic antibodies in Komagataella phaffii (Pichia pastoris) and Escherichia coli for NMR studies
por: Gagné, Donald, et al.
Publicado: (2023)

Intra and Inter-Spore Variability in Rhizophagus irregularis AOX Gene
por: Campos, Catarina, et al.
Publicado: (2015)

Recombinant O-mannosylated protein production (PstS-1) from Mycobacterium tuberculosis in Pichia pastoris (Komagataella phaffii) as a tool to study tuberculosis infection
por: Bando-Campos, Giroshi, et al.
Publicado: (2019)

Identification and characterization of the Komagataella phaffii mating pheromone genes
por: Heistinger, Lina, et al.
Publicado: (2018)

Effect of Methionine on Gene Expression in Komagataella phaffii Cells
por: Ianshina, Tatiana, et al.
Publicado: (2023)

Bioprocess performance analysis of novel methanol-independent promoters for recombinant protein production with Pichia pastoris
por: Garrigós-Martínez, Javier, et al.
Publicado: (2021)

AOX in action—making plant mitochondrial metabolism special
por: Yokoyama, Ryo
Publicado: (2023)

Enabling growth-decoupled Komagataella phaffii recombinant protein production based on the methanol-free P(DH) promoter
por: Bernat-Camps, Núria, et al.
Publicado: (2023)

Clinical implications of TPO and AOX1 in pediatric papillary thyroid carcinoma
por: Yu, Yongbo, et al.
Publicado: (2021)

Pseudohyphal differentiation in Komagataella phaffii: investigating the FLO gene family
por: De, Sonakshi, et al.
Publicado: (2020)

Considerations of AOX Functionality Revealed by Critical Motifs and Unique Domains
por: Brew-Appiah, Rhoda A. T., et al.
Publicado: (2018)

Expression of AOX1 Predicts Prognosis of Clear Cell Renal Cell Carcinoma
por: Xiong, Luyang, et al.
Publicado: (2021)

Alternative Respiratory Pathway Component Genes (AOX and ND) in Rice and Barley and Their Response to Stress
por: Wanniarachchi, Vajira R., et al.
Publicado: (2018)

Transcriptomic Analysis of Pichia pastoris (Komagataella phaffii) GS115 During Heterologous Protein Production Using a High-Cell-Density Fed-Batch Cultivation Strategy
por: Zhang, Chengbo, et al.
Publicado: (2020)

What makes Komagataella phaffii non-conventional?
por: Ata, Özge, et al.
Publicado: (2021)

Cannot write session to /tmp/vufind_sessions/sess_s7scni3s422j7ip3v7prtmk3jt