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Brain-specific Drp1 regulates postsynaptic endocytosis and dendrite formation independently of mitochondrial division
Dynamin-related protein 1 (Drp1) divides mitochondria as a mechano-chemical GTPase. However, the function of Drp1 beyond mitochondrial division is largely unknown. Multiple Drp1 isoforms are produced through mRNA splicing. One such isoform, Drp1(ABCD), contains all four alternative exons and is spec...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6824841/ https://www.ncbi.nlm.nih.gov/pubmed/31603426 http://dx.doi.org/10.7554/eLife.44739 |
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author | Itoh, Kie Murata, Daisuke Kato, Takashi Yamada, Tatsuya Araki, Yoichi Saito, Atsushi Adachi, Yoshihiro Igarashi, Atsushi Li, Shuo Pletnikov, Mikhail Huganir, Richard L Watanabe, Shigeki Kamiya, Atsushi Iijima, Miho Sesaki, Hiromi |
author_facet | Itoh, Kie Murata, Daisuke Kato, Takashi Yamada, Tatsuya Araki, Yoichi Saito, Atsushi Adachi, Yoshihiro Igarashi, Atsushi Li, Shuo Pletnikov, Mikhail Huganir, Richard L Watanabe, Shigeki Kamiya, Atsushi Iijima, Miho Sesaki, Hiromi |
author_sort | Itoh, Kie |
collection | PubMed |
description | Dynamin-related protein 1 (Drp1) divides mitochondria as a mechano-chemical GTPase. However, the function of Drp1 beyond mitochondrial division is largely unknown. Multiple Drp1 isoforms are produced through mRNA splicing. One such isoform, Drp1(ABCD), contains all four alternative exons and is specifically expressed in the brain. Here, we studied the function of Drp1(ABCD) in mouse neurons in both culture and animal systems using isoform-specific knockdown by shRNA and isoform-specific knockout by CRISPR/Cas9. We found that the expression of Drp1(ABCD) is induced during postnatal brain development. Drp1(ABCD) is enriched in dendritic spines and regulates postsynaptic clathrin-mediated endocytosis by positioning the endocytic zone at the postsynaptic density, independently of mitochondrial division. Drp1(ABCD) loss promotes the formation of ectopic dendrites in neurons and enhanced sensorimotor gating behavior in mice. These data reveal that Drp1(ABCD) controls postsynaptic endocytosis, neuronal morphology and brain function. |
format | Online Article Text |
id | pubmed-6824841 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-68248412019-11-04 Brain-specific Drp1 regulates postsynaptic endocytosis and dendrite formation independently of mitochondrial division Itoh, Kie Murata, Daisuke Kato, Takashi Yamada, Tatsuya Araki, Yoichi Saito, Atsushi Adachi, Yoshihiro Igarashi, Atsushi Li, Shuo Pletnikov, Mikhail Huganir, Richard L Watanabe, Shigeki Kamiya, Atsushi Iijima, Miho Sesaki, Hiromi eLife Cell Biology Dynamin-related protein 1 (Drp1) divides mitochondria as a mechano-chemical GTPase. However, the function of Drp1 beyond mitochondrial division is largely unknown. Multiple Drp1 isoforms are produced through mRNA splicing. One such isoform, Drp1(ABCD), contains all four alternative exons and is specifically expressed in the brain. Here, we studied the function of Drp1(ABCD) in mouse neurons in both culture and animal systems using isoform-specific knockdown by shRNA and isoform-specific knockout by CRISPR/Cas9. We found that the expression of Drp1(ABCD) is induced during postnatal brain development. Drp1(ABCD) is enriched in dendritic spines and regulates postsynaptic clathrin-mediated endocytosis by positioning the endocytic zone at the postsynaptic density, independently of mitochondrial division. Drp1(ABCD) loss promotes the formation of ectopic dendrites in neurons and enhanced sensorimotor gating behavior in mice. These data reveal that Drp1(ABCD) controls postsynaptic endocytosis, neuronal morphology and brain function. eLife Sciences Publications, Ltd 2019-10-11 /pmc/articles/PMC6824841/ /pubmed/31603426 http://dx.doi.org/10.7554/eLife.44739 Text en © 2019, Itoh et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Cell Biology Itoh, Kie Murata, Daisuke Kato, Takashi Yamada, Tatsuya Araki, Yoichi Saito, Atsushi Adachi, Yoshihiro Igarashi, Atsushi Li, Shuo Pletnikov, Mikhail Huganir, Richard L Watanabe, Shigeki Kamiya, Atsushi Iijima, Miho Sesaki, Hiromi Brain-specific Drp1 regulates postsynaptic endocytosis and dendrite formation independently of mitochondrial division |
title | Brain-specific Drp1 regulates postsynaptic endocytosis and dendrite formation independently of mitochondrial division |
title_full | Brain-specific Drp1 regulates postsynaptic endocytosis and dendrite formation independently of mitochondrial division |
title_fullStr | Brain-specific Drp1 regulates postsynaptic endocytosis and dendrite formation independently of mitochondrial division |
title_full_unstemmed | Brain-specific Drp1 regulates postsynaptic endocytosis and dendrite formation independently of mitochondrial division |
title_short | Brain-specific Drp1 regulates postsynaptic endocytosis and dendrite formation independently of mitochondrial division |
title_sort | brain-specific drp1 regulates postsynaptic endocytosis and dendrite formation independently of mitochondrial division |
topic | Cell Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6824841/ https://www.ncbi.nlm.nih.gov/pubmed/31603426 http://dx.doi.org/10.7554/eLife.44739 |
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