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Improvement of solubility and yield of recombinant protein expression in E. coli using a two-step system
Overexpression of recombinant proteins in Escherichia coli results in inclusion body formation, and consequently decreased production yield and increased production cost. Co-expression of chaperon systems accompanied by recombinant protein is a general method to increase the production yield. Howeve...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Wolters Kluwer - Medknow
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6827196/ https://www.ncbi.nlm.nih.gov/pubmed/31798656 http://dx.doi.org/10.4103/1735-5362.268200 |