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Accessing Methyl Groups in Proteins via (1)H-detected MAS Solid-state NMR Spectroscopy Employing Random Protonation
We recently introduced RAP (reduced adjoining protonation) labelling as an easy to implement and cost-effective strategy to yield selectively methyl protonated protein samples. We show here that even though the amount of H(2)O employed in the bacterial growth medium is rather low, the intensities ob...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Nature Publishing Group UK
2019
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6828780/ https://www.ncbi.nlm.nih.gov/pubmed/31685894 http://dx.doi.org/10.1038/s41598-019-52383-3 |