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A Chemically Defined Feeder-free System for the Establishment and Maintenance of the Human Naive Pluripotent State

The distinct states of pluripotency in the pre- and post-implantation embryo can be captured in vitro as naive and primed pluripotent stem cell cultures, respectively. The study and application of the naive state remains hampered, particularly in humans, partially due to current culture protocols re...

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Autores principales: Szczerbinska, Iwona, Gonzales, Kevin Andrew Uy, Cukuroglu, Engin, Ramli, Muhammad Nadzim Bin, Lee, Bertha Pei Ge, Tan, Cheng Peow, Wong, Cheng Kit, Rancati, Giulia Irene, Liang, Hongqing, Göke, Jonathan, Ng, Huck-Hui, Chan, Yun-Shen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6829768/
https://www.ncbi.nlm.nih.gov/pubmed/31522974
http://dx.doi.org/10.1016/j.stemcr.2019.08.005
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author Szczerbinska, Iwona
Gonzales, Kevin Andrew Uy
Cukuroglu, Engin
Ramli, Muhammad Nadzim Bin
Lee, Bertha Pei Ge
Tan, Cheng Peow
Wong, Cheng Kit
Rancati, Giulia Irene
Liang, Hongqing
Göke, Jonathan
Ng, Huck-Hui
Chan, Yun-Shen
author_facet Szczerbinska, Iwona
Gonzales, Kevin Andrew Uy
Cukuroglu, Engin
Ramli, Muhammad Nadzim Bin
Lee, Bertha Pei Ge
Tan, Cheng Peow
Wong, Cheng Kit
Rancati, Giulia Irene
Liang, Hongqing
Göke, Jonathan
Ng, Huck-Hui
Chan, Yun-Shen
author_sort Szczerbinska, Iwona
collection PubMed
description The distinct states of pluripotency in the pre- and post-implantation embryo can be captured in vitro as naive and primed pluripotent stem cell cultures, respectively. The study and application of the naive state remains hampered, particularly in humans, partially due to current culture protocols relying on extraneous undefined factors such as feeders. Here we performed a small-molecule screen to identify compounds that facilitate chemically defined establishment and maintenance of human feeder-independent naive embryonic (FINE) stem cells. The expression profile in genic and repetitive elements of FINE cells resembles the 8-cell-to-morula stage in vivo, and only differs from feeder-dependent naive cells in genes involved in cell-cell/cell-matrix interactions. FINE cells offer several technical advantages, such as increased amenability to transfection and a longer period of genomic stability, compared with feeder-dependent cells. Thus, FINE cells will serve as an accessible and useful system for scientific and translational applications of naïve pluripotent stem cells.
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spelling pubmed-68297682019-11-07 A Chemically Defined Feeder-free System for the Establishment and Maintenance of the Human Naive Pluripotent State Szczerbinska, Iwona Gonzales, Kevin Andrew Uy Cukuroglu, Engin Ramli, Muhammad Nadzim Bin Lee, Bertha Pei Ge Tan, Cheng Peow Wong, Cheng Kit Rancati, Giulia Irene Liang, Hongqing Göke, Jonathan Ng, Huck-Hui Chan, Yun-Shen Stem Cell Reports Article The distinct states of pluripotency in the pre- and post-implantation embryo can be captured in vitro as naive and primed pluripotent stem cell cultures, respectively. The study and application of the naive state remains hampered, particularly in humans, partially due to current culture protocols relying on extraneous undefined factors such as feeders. Here we performed a small-molecule screen to identify compounds that facilitate chemically defined establishment and maintenance of human feeder-independent naive embryonic (FINE) stem cells. The expression profile in genic and repetitive elements of FINE cells resembles the 8-cell-to-morula stage in vivo, and only differs from feeder-dependent naive cells in genes involved in cell-cell/cell-matrix interactions. FINE cells offer several technical advantages, such as increased amenability to transfection and a longer period of genomic stability, compared with feeder-dependent cells. Thus, FINE cells will serve as an accessible and useful system for scientific and translational applications of naïve pluripotent stem cells. Elsevier 2019-09-12 /pmc/articles/PMC6829768/ /pubmed/31522974 http://dx.doi.org/10.1016/j.stemcr.2019.08.005 Text en © 2019 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Szczerbinska, Iwona
Gonzales, Kevin Andrew Uy
Cukuroglu, Engin
Ramli, Muhammad Nadzim Bin
Lee, Bertha Pei Ge
Tan, Cheng Peow
Wong, Cheng Kit
Rancati, Giulia Irene
Liang, Hongqing
Göke, Jonathan
Ng, Huck-Hui
Chan, Yun-Shen
A Chemically Defined Feeder-free System for the Establishment and Maintenance of the Human Naive Pluripotent State
title A Chemically Defined Feeder-free System for the Establishment and Maintenance of the Human Naive Pluripotent State
title_full A Chemically Defined Feeder-free System for the Establishment and Maintenance of the Human Naive Pluripotent State
title_fullStr A Chemically Defined Feeder-free System for the Establishment and Maintenance of the Human Naive Pluripotent State
title_full_unstemmed A Chemically Defined Feeder-free System for the Establishment and Maintenance of the Human Naive Pluripotent State
title_short A Chemically Defined Feeder-free System for the Establishment and Maintenance of the Human Naive Pluripotent State
title_sort chemically defined feeder-free system for the establishment and maintenance of the human naive pluripotent state
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6829768/
https://www.ncbi.nlm.nih.gov/pubmed/31522974
http://dx.doi.org/10.1016/j.stemcr.2019.08.005
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