gRNA validation for wheat genome editing with the CRISPR-Cas9 system

BACKGROUND: The CRISPR-Cas9 system is a powerful and versatile tool for crop genome editing. However, achieving highly efficient and specific editing in polyploid species can be a challenge. The efficiency and specificity of the CRISPR-Cas9 system depends critically on the gRNA used. Here, we assess...

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Detalles Bibliográficos
Autores principales: Arndell, Taj, Sharma, Niharika, Langridge, Peter, Baumann, Ute, Watson-Haigh, Nathan S., Whitford, Ryan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6829922/
https://www.ncbi.nlm.nih.gov/pubmed/31684940
http://dx.doi.org/10.1186/s12896-019-0565-z

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6829922/
https://www.ncbi.nlm.nih.gov/pubmed/31684940
http://dx.doi.org/10.1186/s12896-019-0565-z

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