Cargando…

gRNA validation for wheat genome editing with the CRISPR-Cas9 system

BACKGROUND: The CRISPR-Cas9 system is a powerful and versatile tool for crop genome editing. However, achieving highly efficient and specific editing in polyploid species can be a challenge. The efficiency and specificity of the CRISPR-Cas9 system depends critically on the gRNA used. Here, we assess...

Descripción completa

Detalles Bibliográficos
Autores principales:	Arndell, Taj, Sharma, Niharika, Langridge, Peter, Baumann, Ute, Watson-Haigh, Nathan S., Whitford, Ryan
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2019
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6829922/ https://www.ncbi.nlm.nih.gov/pubmed/31684940 http://dx.doi.org/10.1186/s12896-019-0565-z

Ejemplares similares

CRISPR/Cas9‐mediated knockout of Ms1 enables the rapid generation of male‐sterile hexaploid wheat lines for use in hybrid seed production
por: Okada, Anzu, et al.
Publicado: (2019)

Drosophila balancer reengineering using polycistronic gRNA for CRISPR/Cas9 gene editing
por: Benner, Leif, et al.
Publicado: (2018)

Construction and evaluation of gRNA arrays for multiplex CRISPR‐Cas9
por: Žun, Gašper, et al.
Publicado: (2023)

Improved gRNA secondary structures allow editing of target sites resistant to CRISPR-Cas9 cleavage
por: Riesenberg, Stephan, et al.
Publicado: (2022)

The Current State and Future of CRISPR-Cas9 gRNA Design Tools
por: Wilson, Laurence O. W., et al.
Publicado: (2018)

CRISPRon/off: CRISPR/Cas9 on- and off-target gRNA design
por: Anthon, Christian, et al.
Publicado: (2022)

A method to convert mRNA into a gRNA library for CRISPR/Cas9 editing of any organism
por: Arakawa, Hiroshi
Publicado: (2016)

Efficient genome editing using tRNA promoter-driven CRISPR/Cas9 gRNA in Aspergillus niger
por: Song, Letian, et al.
Publicado: (2018)

Programmed gRNA Removal System for CRISPR-Cas9-Mediated Multi-Round Genome Editing in Bacillus subtilis
por: Lim, Hayeon, et al.
Publicado: (2019)

Cas9/gRNA-mediated genome editing of yeast mitochondria and Chlamydomonas chloroplasts
por: Yoo, Byung-Chun, et al.
Publicado: (2020)

Ribozyme Mediated gRNA Generation for In Vitro and In Vivo CRISPR/Cas9 Mutagenesis
por: Lee, Raymond Teck Ho, et al.
Publicado: (2016)

Highly multiplexed genome engineering using CRISPR/Cas9 gRNA arrays
por: Kurata, Morito, et al.
Publicado: (2018)

CRISPR/Cas9-assisted gRNA-free one-step genome editing with no sequence limitations and improved targeting efficiency
por: Zhao, Dongdong, et al.
Publicado: (2017)

A gRNA-tRNA array for CRISPR-Cas9 based rapid multiplexed genome editing in Saccharomyces cerevisiae
por: Zhang, Yueping, et al.
Publicado: (2019)

Rapid and efficient one-step generation of paired gRNA CRISPR-Cas9 libraries
por: Vidigal, Joana A., et al.
Publicado: (2015)

Versatile single-step-assembly CRISPR/Cas9 vectors for dual gRNA expression
por: Adikusuma, Fatwa, et al.
Publicado: (2017)

Synthetic Circular gRNA Mediated Biological Function of CRISPR-(d)Cas9 System
por: Wang, Mingxia, et al.
Publicado: (2022)

Enhancing CRISPR-Cas9 gRNA efficiency prediction by data integration and deep learning
por: Xiang, Xi, et al.
Publicado: (2021)

The gRNA-miRNA-gRNA Ternary Cassette Combining CRISPR/Cas9 with RNAi Approach Strongly Inhibits Hepatitis B Virus Replication
por: Wang, Jie, et al.
Publicado: (2017)

Genome dependent Cas9/gRNA search time underlies sequence dependent gRNA activity
por: Moreb, E. A., et al.
Publicado: (2021)

The Development of a Viral Mediated CRISPR/Cas9 System with Doxycycline Dependent gRNA Expression for Inducible In vitro and In vivo Genome Editing
por: de Solis, Christopher A., et al.
Publicado: (2016)

Development of a gRNA Expression and Processing Platform for Efficient CRISPR-Cas9-Based Gene Editing and Gene Silencing in Candida tropicalis
por: Li, Yujie, et al.
Publicado: (2022)

CRISPR–Cas9 gRNA efficiency prediction: an overview of predictive tools and the role of deep learning
por: Konstantakos, Vasileios, et al.
Publicado: (2022)

CRISPR/Cas9 gRNA activity depends on free energy changes and on the target PAM context
por: Corsi, Giulia I., et al.
Publicado: (2022)

In Planta Processing of the SpCas9–gRNA Complex
por: Mikami, Masafumi, et al.
Publicado: (2017)

From design to validation of CRISPR/gRNA primers towards genome editing
por: Jamee, Mohd Rizwan, et al.
Publicado: (2022)

Comparison and optimization of CRISPR/dCas9/gRNA genome-labeling systems for live cell imaging
por: Hong, Yu, et al.
Publicado: (2018)

CSC software corrects off-target mediated gRNA depletion in CRISPR-Cas9 essentiality screens
por: Perez, Alexendar R., et al.
Publicado: (2021)

Screening of CRISPR/Cas9 gRNA for mimicking Powdery Mildew resistant MLO ol-2 mutant
por: Prajapati, Archana, et al.
Publicado: (2021)

Versatile CRISPR/Cas9-mediated mosaic analysis by gRNA-induced crossing-over for unmodified genomes
por: Allen, Sarah E., et al.
Publicado: (2021)

An in-library ligation strategy and its application in CRISPR/Cas9 screening of high-order gRNA combinations
por: Lu, Zhike, et al.
Publicado: (2022)

Study of the effect of the introduction of mitochondrial import determinants into the gRNA structure on the activity of the gRNA/SpCas9 complex in vitro
por: Zakirova, E.G., et al.
Publicado: (2020)

CRISPR-Cas9 Dual-gRNA Attack Causes Mutation, Excision and Inversion of the HIV-1 Proviral DNA
por: Binda, Caroline S., et al.
Publicado: (2020)

The Cas9-gRNA ribonucleoprotein complex-mediated editing of pyrG in Ganoderma lucidum and unexpected insertion of contaminated DNA fragments
por: Eom, Hyerang, et al.
Publicado: (2023)

One step generation of customizable gRNA vectors for multiplex CRISPR approaches through string assembly gRNA cloning (STAgR)
por: Breunig, Christopher T., et al.
Publicado: (2018)

Synthetic gRNA/Cas9 Ribonucleoprotein Inhibits HIV Reactivation and Replication
por: Khanal, Sushant, et al.
Publicado: (2022)

Efficient multiplex genome editing by CRISPR/Cas9 in common wheat
por: Li, Jihu, et al.
Publicado: (2020)

Uncertainty-aware and interpretable evaluation of Cas9–gRNA and Cas12a–gRNA specificity for fully matched and partially mismatched targets with Deep Kernel Learning
por: Kirillov, Bogdan, et al.
Publicado: (2021)

Targeted delivery of CRISPR/Cas9 to prostate cancer by modified gRNA using a flexible aptamer-cationic liposome
por: Zhen, Shuai, et al.
Publicado: (2016)

5′ modifications to CRISPR–Cas9 gRNA can change the dynamics and size of R-loops and inhibit DNA cleavage
por: Mullally, Grace, et al.
Publicado: (2020)

Cannot write session to /tmp/vufind_sessions/sess_ve95ll2g9ngm2lpesf6c06qbpq