Matrine induces apoptosis via targeting CCR7 and enhances the effect of anticancer drugs in non-small cell lung cancer in vitro

This study mainly investigated the effects of matrine on cell apoptosis and the effects of anticancer drugs in non-small cell lung cancer (NSCLC) cell lines (A549 and LK2 cells). The results showed that matrine (≥10 μM) caused a significant inhibition on cell viability and 10 and 100 μM matrine indu...

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Autores principales: Pu, Jiangtao, Tang, Xiaojun, Zhuang, Xiang, Hu, Zhi, He, Kaiming, Wu, Yunfei, Dai, Tianyang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2018
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Original Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6830874/
https://www.ncbi.nlm.nih.gov/pubmed/30236029
http://dx.doi.org/10.1177/1753425918800555
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author Pu, Jiangtao
Tang, Xiaojun
Zhuang, Xiang
Hu, Zhi
He, Kaiming
Wu, Yunfei
Dai, Tianyang
author_facet Pu, Jiangtao
Tang, Xiaojun
Zhuang, Xiang
Hu, Zhi
He, Kaiming
Wu, Yunfei
Dai, Tianyang
author_sort Pu, Jiangtao
collection PubMed
description This study mainly investigated the effects of matrine on cell apoptosis and the effects of anticancer drugs in non-small cell lung cancer (NSCLC) cell lines (A549 and LK2 cells). The results showed that matrine (≥10 μM) caused a significant inhibition on cell viability and 10 and 100 μM matrine induced cell apoptosis via influencing p53, bax, casp3, and bcl-2 expressions in A549 cells. In addition, matrine significantly down-regulated C-C chemokine receptor type 7 (CCR7) expression, and blocking the down-regulation of CCR7 by exogenous chemokine ligand 21 (CCL21) treatment alleviated matrine-caused effects of apoptosis genes in A549 cells. The results were further validated in LK2 cells that matrine regulated apoptosis gene expressions, which were reversed by CCL21 treatment. Furthermore, matrine enhances the effects of cisplatin, 5-fluorouracil, and paclitaxel in A549 cells, and the anticancer effects exhibit a dosage-dependent manner. In summary, matrine induced cell apoptosis and enhanced the effects of anticancer drugs in NSCLC cells; the mechanism might be associated with the CCR7 signal.
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spelling pubmed-68308742019-11-20 Matrine induces apoptosis via targeting CCR7 and enhances the effect of anticancer drugs in non-small cell lung cancer in vitro Pu, Jiangtao Tang, Xiaojun Zhuang, Xiang Hu, Zhi He, Kaiming Wu, Yunfei Dai, Tianyang Innate Immun Original Articles This study mainly investigated the effects of matrine on cell apoptosis and the effects of anticancer drugs in non-small cell lung cancer (NSCLC) cell lines (A549 and LK2 cells). The results showed that matrine (≥10 μM) caused a significant inhibition on cell viability and 10 and 100 μM matrine induced cell apoptosis via influencing p53, bax, casp3, and bcl-2 expressions in A549 cells. In addition, matrine significantly down-regulated C-C chemokine receptor type 7 (CCR7) expression, and blocking the down-regulation of CCR7 by exogenous chemokine ligand 21 (CCL21) treatment alleviated matrine-caused effects of apoptosis genes in A549 cells. The results were further validated in LK2 cells that matrine regulated apoptosis gene expressions, which were reversed by CCL21 treatment. Furthermore, matrine enhances the effects of cisplatin, 5-fluorouracil, and paclitaxel in A549 cells, and the anticancer effects exhibit a dosage-dependent manner. In summary, matrine induced cell apoptosis and enhanced the effects of anticancer drugs in NSCLC cells; the mechanism might be associated with the CCR7 signal. SAGE Publications 2018-09-20 2018-10 /pmc/articles/PMC6830874/ /pubmed/30236029 http://dx.doi.org/10.1177/1753425918800555 Text en © The Author(s) 2018 http://creativecommons.org/licenses/by-nc/4.0/ Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Original Articles
Pu, Jiangtao
Tang, Xiaojun
Zhuang, Xiang
Hu, Zhi
He, Kaiming
Wu, Yunfei
Dai, Tianyang
Matrine induces apoptosis via targeting CCR7 and enhances the effect of anticancer drugs in non-small cell lung cancer in vitro
title Matrine induces apoptosis via targeting CCR7 and enhances the effect of anticancer drugs in non-small cell lung cancer in vitro
title_full Matrine induces apoptosis via targeting CCR7 and enhances the effect of anticancer drugs in non-small cell lung cancer in vitro
title_fullStr Matrine induces apoptosis via targeting CCR7 and enhances the effect of anticancer drugs in non-small cell lung cancer in vitro
title_full_unstemmed Matrine induces apoptosis via targeting CCR7 and enhances the effect of anticancer drugs in non-small cell lung cancer in vitro
title_short Matrine induces apoptosis via targeting CCR7 and enhances the effect of anticancer drugs in non-small cell lung cancer in vitro
title_sort matrine induces apoptosis via targeting ccr7 and enhances the effect of anticancer drugs in non-small cell lung cancer in vitro
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6830874/
https://www.ncbi.nlm.nih.gov/pubmed/30236029
http://dx.doi.org/10.1177/1753425918800555
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