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Metabolic engineering and transcriptomic analysis of Saccharomyces cerevisiae producing p-coumaric acid from xylose

BACKGROUND: Aromatic amino acids and their derivatives are valuable chemicals and are precursors for different industrially compounds. p-Coumaric acid is the main building block for complex secondary metabolites in commercial demand, such as flavonoids and polyphenols. Industrial scale production of...

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Autores principales: Borja, Gheorghe M., Rodriguez, Angelica, Campbell, Kate, Borodina, Irina, Chen, Yun, Nielsen, Jens
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6833135/
https://www.ncbi.nlm.nih.gov/pubmed/31690329
http://dx.doi.org/10.1186/s12934-019-1244-4
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author Borja, Gheorghe M.
Rodriguez, Angelica
Campbell, Kate
Borodina, Irina
Chen, Yun
Nielsen, Jens
author_facet Borja, Gheorghe M.
Rodriguez, Angelica
Campbell, Kate
Borodina, Irina
Chen, Yun
Nielsen, Jens
author_sort Borja, Gheorghe M.
collection PubMed
description BACKGROUND: Aromatic amino acids and their derivatives are valuable chemicals and are precursors for different industrially compounds. p-Coumaric acid is the main building block for complex secondary metabolites in commercial demand, such as flavonoids and polyphenols. Industrial scale production of this compound from yeast however remains challenging. RESULTS: Using metabolic engineering and a systems biology approach, we developed a Saccharomyces cerevisiae platform strain able to produce 242 mg/L of p-coumaric acid from xylose. The same strain produced only 5.35 mg/L when cultivated with glucose as carbon source. To characterise this platform strain further, transcriptomic analysis was performed, comparing this strain’s growth on xylose and glucose, revealing a strong up-regulation of the glyoxylate pathway alongside increased cell wall biosynthesis and unexpectedly a decrease in aromatic amino acid gene expression when xylose was used as carbon source. CONCLUSIONS: The resulting S. cerevisiae strain represents a promising platform host for future production of p-coumaric using xylose as a carbon source.
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spelling pubmed-68331352019-11-08 Metabolic engineering and transcriptomic analysis of Saccharomyces cerevisiae producing p-coumaric acid from xylose Borja, Gheorghe M. Rodriguez, Angelica Campbell, Kate Borodina, Irina Chen, Yun Nielsen, Jens Microb Cell Fact Research BACKGROUND: Aromatic amino acids and their derivatives are valuable chemicals and are precursors for different industrially compounds. p-Coumaric acid is the main building block for complex secondary metabolites in commercial demand, such as flavonoids and polyphenols. Industrial scale production of this compound from yeast however remains challenging. RESULTS: Using metabolic engineering and a systems biology approach, we developed a Saccharomyces cerevisiae platform strain able to produce 242 mg/L of p-coumaric acid from xylose. The same strain produced only 5.35 mg/L when cultivated with glucose as carbon source. To characterise this platform strain further, transcriptomic analysis was performed, comparing this strain’s growth on xylose and glucose, revealing a strong up-regulation of the glyoxylate pathway alongside increased cell wall biosynthesis and unexpectedly a decrease in aromatic amino acid gene expression when xylose was used as carbon source. CONCLUSIONS: The resulting S. cerevisiae strain represents a promising platform host for future production of p-coumaric using xylose as a carbon source. BioMed Central 2019-11-05 /pmc/articles/PMC6833135/ /pubmed/31690329 http://dx.doi.org/10.1186/s12934-019-1244-4 Text en © The Author(s) 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Borja, Gheorghe M.
Rodriguez, Angelica
Campbell, Kate
Borodina, Irina
Chen, Yun
Nielsen, Jens
Metabolic engineering and transcriptomic analysis of Saccharomyces cerevisiae producing p-coumaric acid from xylose
title Metabolic engineering and transcriptomic analysis of Saccharomyces cerevisiae producing p-coumaric acid from xylose
title_full Metabolic engineering and transcriptomic analysis of Saccharomyces cerevisiae producing p-coumaric acid from xylose
title_fullStr Metabolic engineering and transcriptomic analysis of Saccharomyces cerevisiae producing p-coumaric acid from xylose
title_full_unstemmed Metabolic engineering and transcriptomic analysis of Saccharomyces cerevisiae producing p-coumaric acid from xylose
title_short Metabolic engineering and transcriptomic analysis of Saccharomyces cerevisiae producing p-coumaric acid from xylose
title_sort metabolic engineering and transcriptomic analysis of saccharomyces cerevisiae producing p-coumaric acid from xylose
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6833135/
https://www.ncbi.nlm.nih.gov/pubmed/31690329
http://dx.doi.org/10.1186/s12934-019-1244-4
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