Cargando…

Comparative Study of Extracellular Proteolytic, Cellulolytic, and Hemicellulolytic Enzyme Activities and Biotransformation of Palm Kernel Cake Biomass by Lactic Acid Bacteria Isolated from Malaysian Foods

Biotransformation via solid state fermentation (SSF) mediated by microorganisms is a promising approach to produce useful products from agricultural biomass. Lactic acid bacteria (LAB) that are commonly found in fermented foods have been shown to exhibit extracellular proteolytic, β-glucosidase, β-m...

Descripción completa

Detalles Bibliográficos
Autores principales: Lee, Fu Haw, Wan, Suet Ying, Foo, Hooi Ling, Loh, Teck Chwen, Mohamad, Rosfarizan, Abdul Rahim, Raha, Idrus, Zulkifli
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6834149/
https://www.ncbi.nlm.nih.gov/pubmed/31600952
http://dx.doi.org/10.3390/ijms20204979
_version_ 1783466430274994176
author Lee, Fu Haw
Wan, Suet Ying
Foo, Hooi Ling
Loh, Teck Chwen
Mohamad, Rosfarizan
Abdul Rahim, Raha
Idrus, Zulkifli
author_facet Lee, Fu Haw
Wan, Suet Ying
Foo, Hooi Ling
Loh, Teck Chwen
Mohamad, Rosfarizan
Abdul Rahim, Raha
Idrus, Zulkifli
author_sort Lee, Fu Haw
collection PubMed
description Biotransformation via solid state fermentation (SSF) mediated by microorganisms is a promising approach to produce useful products from agricultural biomass. Lactic acid bacteria (LAB) that are commonly found in fermented foods have been shown to exhibit extracellular proteolytic, β-glucosidase, β-mannosidase, and β-mannanase activities. Therefore, extracellular proteolytic, cellulolytic, and hemicellulolytic enzyme activities of seven Lactobacillus plantarum strains (a prominent species of LAB) isolated from Malaysian foods were compared in this study. The biotransformation of palm kernel cake (PKC) biomass mediated by selected L. plantarum strains was subsequently conducted. The results obtained in this study exhibited the studied L. plantarum strains produced versatile multi extracellular hydrolytic enzyme activities that were active from acidic to alkaline pH conditions. The highest total score of extracellular hydrolytic enzyme activities were recorded by L. plantarum RI11, L. plantarum RG11, and L. plantarum RG14. Therefore, they were selected for the subsequent biotransformation of PKC biomass via SSF. The hydrolytic enzyme activities of treated PKC extract were compared for each sampling interval. The scanning electron microscopy analyses revealed the formation of extracellular matrices around L. plantarum strains attached to the surface of PKC biomass during SSF, inferring that the investigated L. plantarum strains have the capability to grow on PKC biomass and perform synergistic secretions of various extracellular proteolytic, cellulolytic, and hemicellulolytic enzymes that were essential for the effective biodegradation of PKC. The substantial growth of selected L. plamtraum strains on PKC during SSF revealed the promising application of selected L. plantarum strains as a biotransformation agent for cellulosic biomass.
format Online
Article
Text
id pubmed-6834149
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-68341492019-11-25 Comparative Study of Extracellular Proteolytic, Cellulolytic, and Hemicellulolytic Enzyme Activities and Biotransformation of Palm Kernel Cake Biomass by Lactic Acid Bacteria Isolated from Malaysian Foods Lee, Fu Haw Wan, Suet Ying Foo, Hooi Ling Loh, Teck Chwen Mohamad, Rosfarizan Abdul Rahim, Raha Idrus, Zulkifli Int J Mol Sci Article Biotransformation via solid state fermentation (SSF) mediated by microorganisms is a promising approach to produce useful products from agricultural biomass. Lactic acid bacteria (LAB) that are commonly found in fermented foods have been shown to exhibit extracellular proteolytic, β-glucosidase, β-mannosidase, and β-mannanase activities. Therefore, extracellular proteolytic, cellulolytic, and hemicellulolytic enzyme activities of seven Lactobacillus plantarum strains (a prominent species of LAB) isolated from Malaysian foods were compared in this study. The biotransformation of palm kernel cake (PKC) biomass mediated by selected L. plantarum strains was subsequently conducted. The results obtained in this study exhibited the studied L. plantarum strains produced versatile multi extracellular hydrolytic enzyme activities that were active from acidic to alkaline pH conditions. The highest total score of extracellular hydrolytic enzyme activities were recorded by L. plantarum RI11, L. plantarum RG11, and L. plantarum RG14. Therefore, they were selected for the subsequent biotransformation of PKC biomass via SSF. The hydrolytic enzyme activities of treated PKC extract were compared for each sampling interval. The scanning electron microscopy analyses revealed the formation of extracellular matrices around L. plantarum strains attached to the surface of PKC biomass during SSF, inferring that the investigated L. plantarum strains have the capability to grow on PKC biomass and perform synergistic secretions of various extracellular proteolytic, cellulolytic, and hemicellulolytic enzymes that were essential for the effective biodegradation of PKC. The substantial growth of selected L. plamtraum strains on PKC during SSF revealed the promising application of selected L. plantarum strains as a biotransformation agent for cellulosic biomass. MDPI 2019-10-09 /pmc/articles/PMC6834149/ /pubmed/31600952 http://dx.doi.org/10.3390/ijms20204979 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lee, Fu Haw
Wan, Suet Ying
Foo, Hooi Ling
Loh, Teck Chwen
Mohamad, Rosfarizan
Abdul Rahim, Raha
Idrus, Zulkifli
Comparative Study of Extracellular Proteolytic, Cellulolytic, and Hemicellulolytic Enzyme Activities and Biotransformation of Palm Kernel Cake Biomass by Lactic Acid Bacteria Isolated from Malaysian Foods
title Comparative Study of Extracellular Proteolytic, Cellulolytic, and Hemicellulolytic Enzyme Activities and Biotransformation of Palm Kernel Cake Biomass by Lactic Acid Bacteria Isolated from Malaysian Foods
title_full Comparative Study of Extracellular Proteolytic, Cellulolytic, and Hemicellulolytic Enzyme Activities and Biotransformation of Palm Kernel Cake Biomass by Lactic Acid Bacteria Isolated from Malaysian Foods
title_fullStr Comparative Study of Extracellular Proteolytic, Cellulolytic, and Hemicellulolytic Enzyme Activities and Biotransformation of Palm Kernel Cake Biomass by Lactic Acid Bacteria Isolated from Malaysian Foods
title_full_unstemmed Comparative Study of Extracellular Proteolytic, Cellulolytic, and Hemicellulolytic Enzyme Activities and Biotransformation of Palm Kernel Cake Biomass by Lactic Acid Bacteria Isolated from Malaysian Foods
title_short Comparative Study of Extracellular Proteolytic, Cellulolytic, and Hemicellulolytic Enzyme Activities and Biotransformation of Palm Kernel Cake Biomass by Lactic Acid Bacteria Isolated from Malaysian Foods
title_sort comparative study of extracellular proteolytic, cellulolytic, and hemicellulolytic enzyme activities and biotransformation of palm kernel cake biomass by lactic acid bacteria isolated from malaysian foods
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6834149/
https://www.ncbi.nlm.nih.gov/pubmed/31600952
http://dx.doi.org/10.3390/ijms20204979
work_keys_str_mv AT leefuhaw comparativestudyofextracellularproteolyticcellulolyticandhemicellulolyticenzymeactivitiesandbiotransformationofpalmkernelcakebiomassbylacticacidbacteriaisolatedfrommalaysianfoods
AT wansuetying comparativestudyofextracellularproteolyticcellulolyticandhemicellulolyticenzymeactivitiesandbiotransformationofpalmkernelcakebiomassbylacticacidbacteriaisolatedfrommalaysianfoods
AT foohooiling comparativestudyofextracellularproteolyticcellulolyticandhemicellulolyticenzymeactivitiesandbiotransformationofpalmkernelcakebiomassbylacticacidbacteriaisolatedfrommalaysianfoods
AT lohteckchwen comparativestudyofextracellularproteolyticcellulolyticandhemicellulolyticenzymeactivitiesandbiotransformationofpalmkernelcakebiomassbylacticacidbacteriaisolatedfrommalaysianfoods
AT mohamadrosfarizan comparativestudyofextracellularproteolyticcellulolyticandhemicellulolyticenzymeactivitiesandbiotransformationofpalmkernelcakebiomassbylacticacidbacteriaisolatedfrommalaysianfoods
AT abdulrahimraha comparativestudyofextracellularproteolyticcellulolyticandhemicellulolyticenzymeactivitiesandbiotransformationofpalmkernelcakebiomassbylacticacidbacteriaisolatedfrommalaysianfoods
AT idruszulkifli comparativestudyofextracellularproteolyticcellulolyticandhemicellulolyticenzymeactivitiesandbiotransformationofpalmkernelcakebiomassbylacticacidbacteriaisolatedfrommalaysianfoods