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Microbial Production of the Off-Flavor Geosmin in Tilapia Production in Brazilian Water Reservoirs: Importance of Bacteria in the Intestine and Other Fish-Associated Environments

AIM: To determine major sources of microbially produced geosmin in the commercially important aquaculture fish species tilapia. METHODS AND RESULTS: Abundance and composition of geosmin-producing bacteria in water and fish biosphere (intestine, digesta, and fins) of Nile tilapia (Oreachromis nilotic...

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Autores principales: Lukassen, Mie B., de Jonge, Nadieh, Bjerregaard, Sabine M., Podduturi, Raju, Jørgensen, Niels O. G., Petersen, Mikael A., David, Gianmarco S., da Silva, Reinaldo J., Nielsen, Jeppe L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6835154/
https://www.ncbi.nlm.nih.gov/pubmed/31736894
http://dx.doi.org/10.3389/fmicb.2019.02447
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author Lukassen, Mie B.
de Jonge, Nadieh
Bjerregaard, Sabine M.
Podduturi, Raju
Jørgensen, Niels O. G.
Petersen, Mikael A.
David, Gianmarco S.
da Silva, Reinaldo J.
Nielsen, Jeppe L.
author_facet Lukassen, Mie B.
de Jonge, Nadieh
Bjerregaard, Sabine M.
Podduturi, Raju
Jørgensen, Niels O. G.
Petersen, Mikael A.
David, Gianmarco S.
da Silva, Reinaldo J.
Nielsen, Jeppe L.
author_sort Lukassen, Mie B.
collection PubMed
description AIM: To determine major sources of microbially produced geosmin in the commercially important aquaculture fish species tilapia. METHODS AND RESULTS: Abundance and composition of geosmin-producing bacteria in water and fish biosphere (intestine, digesta, and fins) of Nile tilapia (Oreachromis niloticus) raised in net cages in Brazilian freshwater farms were examined. By combining qPCR of the geosmin synthase geoA gene and 16S rRNA gene amplicon sequencing to identify potential geosmin-producing organisms, we observed that the proportion and composition of geosmin producers appeared to be rather similar in the water, digesta, intestinal mucous, and on skin, making up about 0.1–0.2% of the total bacterial densities. A high proportion of Cyanobacteria and other putative geosmin producers affiliated to the Actinomycetales were identified in the intestinal mucous layer. The main uptake site for geosmin in fish is traditionally assumed to be through the gill surface, but the present results suggest that uptake by the intestinal tract may represent a major source of geosmin uptake in fish. CONCLUSION: The high abundance of geosmin-producing bacteria in the intestinal mucous layer and digesta may indicate that the digestive system in fish is an important, but hitherto overlooked, source of geosmin and likely other off-flavors in fish. SIGNIFICANCE AND IMPACT OF STUDY: Tainting of fish by microbially produced off-flavors spoils fish quality and lowers consumer preferences for aquaculture-produced freshwater fish. Our results highlight the potential for the application of probiotic microorganisms for management of the intestinal microflora to improve the fish quality. HIGHLIGHTS: -. Off-flavor producing bacteria are widely abundant in aquaculture. -. Off-flavor producers found on skin surface of fish. -. Off-flavor producing bacteria accumulate in the digestive system. -. Off-flavor producers might release significant amounts of off-flavor during lysis in the gut. -. Off-flavor uptake through the digestive system might be quantitatively significant.
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spelling pubmed-68351542019-11-15 Microbial Production of the Off-Flavor Geosmin in Tilapia Production in Brazilian Water Reservoirs: Importance of Bacteria in the Intestine and Other Fish-Associated Environments Lukassen, Mie B. de Jonge, Nadieh Bjerregaard, Sabine M. Podduturi, Raju Jørgensen, Niels O. G. Petersen, Mikael A. David, Gianmarco S. da Silva, Reinaldo J. Nielsen, Jeppe L. Front Microbiol Microbiology AIM: To determine major sources of microbially produced geosmin in the commercially important aquaculture fish species tilapia. METHODS AND RESULTS: Abundance and composition of geosmin-producing bacteria in water and fish biosphere (intestine, digesta, and fins) of Nile tilapia (Oreachromis niloticus) raised in net cages in Brazilian freshwater farms were examined. By combining qPCR of the geosmin synthase geoA gene and 16S rRNA gene amplicon sequencing to identify potential geosmin-producing organisms, we observed that the proportion and composition of geosmin producers appeared to be rather similar in the water, digesta, intestinal mucous, and on skin, making up about 0.1–0.2% of the total bacterial densities. A high proportion of Cyanobacteria and other putative geosmin producers affiliated to the Actinomycetales were identified in the intestinal mucous layer. The main uptake site for geosmin in fish is traditionally assumed to be through the gill surface, but the present results suggest that uptake by the intestinal tract may represent a major source of geosmin uptake in fish. CONCLUSION: The high abundance of geosmin-producing bacteria in the intestinal mucous layer and digesta may indicate that the digestive system in fish is an important, but hitherto overlooked, source of geosmin and likely other off-flavors in fish. SIGNIFICANCE AND IMPACT OF STUDY: Tainting of fish by microbially produced off-flavors spoils fish quality and lowers consumer preferences for aquaculture-produced freshwater fish. Our results highlight the potential for the application of probiotic microorganisms for management of the intestinal microflora to improve the fish quality. HIGHLIGHTS: -. Off-flavor producing bacteria are widely abundant in aquaculture. -. Off-flavor producers found on skin surface of fish. -. Off-flavor producing bacteria accumulate in the digestive system. -. Off-flavor producers might release significant amounts of off-flavor during lysis in the gut. -. Off-flavor uptake through the digestive system might be quantitatively significant. Frontiers Media S.A. 2019-10-31 /pmc/articles/PMC6835154/ /pubmed/31736894 http://dx.doi.org/10.3389/fmicb.2019.02447 Text en Copyright © 2019 Lukassen, de Jonge, Bjerregaard, Podduturi, Jørgensen, Petersen, David, da Silva and Nielsen. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Lukassen, Mie B.
de Jonge, Nadieh
Bjerregaard, Sabine M.
Podduturi, Raju
Jørgensen, Niels O. G.
Petersen, Mikael A.
David, Gianmarco S.
da Silva, Reinaldo J.
Nielsen, Jeppe L.
Microbial Production of the Off-Flavor Geosmin in Tilapia Production in Brazilian Water Reservoirs: Importance of Bacteria in the Intestine and Other Fish-Associated Environments
title Microbial Production of the Off-Flavor Geosmin in Tilapia Production in Brazilian Water Reservoirs: Importance of Bacteria in the Intestine and Other Fish-Associated Environments
title_full Microbial Production of the Off-Flavor Geosmin in Tilapia Production in Brazilian Water Reservoirs: Importance of Bacteria in the Intestine and Other Fish-Associated Environments
title_fullStr Microbial Production of the Off-Flavor Geosmin in Tilapia Production in Brazilian Water Reservoirs: Importance of Bacteria in the Intestine and Other Fish-Associated Environments
title_full_unstemmed Microbial Production of the Off-Flavor Geosmin in Tilapia Production in Brazilian Water Reservoirs: Importance of Bacteria in the Intestine and Other Fish-Associated Environments
title_short Microbial Production of the Off-Flavor Geosmin in Tilapia Production in Brazilian Water Reservoirs: Importance of Bacteria in the Intestine and Other Fish-Associated Environments
title_sort microbial production of the off-flavor geosmin in tilapia production in brazilian water reservoirs: importance of bacteria in the intestine and other fish-associated environments
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6835154/
https://www.ncbi.nlm.nih.gov/pubmed/31736894
http://dx.doi.org/10.3389/fmicb.2019.02447
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