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The Antimicrobial and Antiviral Activity of Polyphenols from Almond (Prunus dulcis L.) Skin

Due to their antimicrobial and antiviral activity potential in vitro, polyphenols are gaining a lot of attention from the pharmaceutical and healthcare industries. A novel antiviral and antimicrobial approach could be based on the use of polyphenols obtained from natural sources. Here, we tested the...

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Autores principales: Musarra-Pizzo, Maria, Ginestra, Giovanna, Smeriglio, Antonella, Pennisi, Rosamaria, Sciortino, Maria Teresa, Mandalari, Giuseppina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6836111/
https://www.ncbi.nlm.nih.gov/pubmed/31623329
http://dx.doi.org/10.3390/nu11102355
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author Musarra-Pizzo, Maria
Ginestra, Giovanna
Smeriglio, Antonella
Pennisi, Rosamaria
Sciortino, Maria Teresa
Mandalari, Giuseppina
author_facet Musarra-Pizzo, Maria
Ginestra, Giovanna
Smeriglio, Antonella
Pennisi, Rosamaria
Sciortino, Maria Teresa
Mandalari, Giuseppina
author_sort Musarra-Pizzo, Maria
collection PubMed
description Due to their antimicrobial and antiviral activity potential in vitro, polyphenols are gaining a lot of attention from the pharmaceutical and healthcare industries. A novel antiviral and antimicrobial approach could be based on the use of polyphenols obtained from natural sources. Here, we tested the antibacterial and antiviral effect of a mix of polyphenols present in natural almond skin (NS MIX). The antimicrobial potential was evaluated against the standard American Type Culture Collection (ATCC) and clinical strains of Staphylococcus aureus, including methicillin-resistant (MRSA) strains, by minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). Herpes simplex virus type I was used for the antiviral assessment of NS MIX by plaque assay. Furthermore, we evaluated the expression of viral cascade antigens. NS MIX exhibited antimicrobial (MIC values of 0.31–1.25 mg/ml) and antiviral activity (decrease in the viral titer ** p < 0.01, and viral DNA accumulation * p < 0.05) against Staphylococcus aureus and HSV-1, respectively. Amongst the isolated compounds, the aglycones epicatechin and catechin showed the greatest activity against S. aureus ATCC 6538P (MIC values of 0.078–0.15 and 0.15 mg/ml, respectively), but were not active against all the other strains. These results could be used to develop novel products for topical use.
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spelling pubmed-68361112019-11-25 The Antimicrobial and Antiviral Activity of Polyphenols from Almond (Prunus dulcis L.) Skin Musarra-Pizzo, Maria Ginestra, Giovanna Smeriglio, Antonella Pennisi, Rosamaria Sciortino, Maria Teresa Mandalari, Giuseppina Nutrients Article Due to their antimicrobial and antiviral activity potential in vitro, polyphenols are gaining a lot of attention from the pharmaceutical and healthcare industries. A novel antiviral and antimicrobial approach could be based on the use of polyphenols obtained from natural sources. Here, we tested the antibacterial and antiviral effect of a mix of polyphenols present in natural almond skin (NS MIX). The antimicrobial potential was evaluated against the standard American Type Culture Collection (ATCC) and clinical strains of Staphylococcus aureus, including methicillin-resistant (MRSA) strains, by minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). Herpes simplex virus type I was used for the antiviral assessment of NS MIX by plaque assay. Furthermore, we evaluated the expression of viral cascade antigens. NS MIX exhibited antimicrobial (MIC values of 0.31–1.25 mg/ml) and antiviral activity (decrease in the viral titer ** p < 0.01, and viral DNA accumulation * p < 0.05) against Staphylococcus aureus and HSV-1, respectively. Amongst the isolated compounds, the aglycones epicatechin and catechin showed the greatest activity against S. aureus ATCC 6538P (MIC values of 0.078–0.15 and 0.15 mg/ml, respectively), but were not active against all the other strains. These results could be used to develop novel products for topical use. MDPI 2019-10-03 /pmc/articles/PMC6836111/ /pubmed/31623329 http://dx.doi.org/10.3390/nu11102355 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Musarra-Pizzo, Maria
Ginestra, Giovanna
Smeriglio, Antonella
Pennisi, Rosamaria
Sciortino, Maria Teresa
Mandalari, Giuseppina
The Antimicrobial and Antiviral Activity of Polyphenols from Almond (Prunus dulcis L.) Skin
title The Antimicrobial and Antiviral Activity of Polyphenols from Almond (Prunus dulcis L.) Skin
title_full The Antimicrobial and Antiviral Activity of Polyphenols from Almond (Prunus dulcis L.) Skin
title_fullStr The Antimicrobial and Antiviral Activity of Polyphenols from Almond (Prunus dulcis L.) Skin
title_full_unstemmed The Antimicrobial and Antiviral Activity of Polyphenols from Almond (Prunus dulcis L.) Skin
title_short The Antimicrobial and Antiviral Activity of Polyphenols from Almond (Prunus dulcis L.) Skin
title_sort antimicrobial and antiviral activity of polyphenols from almond (prunus dulcis l.) skin
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6836111/
https://www.ncbi.nlm.nih.gov/pubmed/31623329
http://dx.doi.org/10.3390/nu11102355
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