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Gene expression profiling of single cells from archival tissue with laser-capture microdissection and Smart-3SEQ

RNA sequencing (RNA-seq) is a sensitive and accurate method for quantifying gene expression. Small samples or those whose RNA is degraded, such as formalin-fixed paraffin-embedded (FFPE) tissue, remain challenging to study with nonspecialized RNA-seq protocols. Here, we present a new method, Smart-3...

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Detalles Bibliográficos
Autores principales: Foley, Joseph W., Zhu, Chunfang, Jolivet, Philippe, Zhu, Shirley X., Lu, Peipei, Meaney, Michael J., West, Robert B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6836736/
https://www.ncbi.nlm.nih.gov/pubmed/31519740
http://dx.doi.org/10.1101/gr.234807.118