Improvement of Soluble Production of Reteplase in Escherichia coli by Optimization of Chemical Chaperones in Lysis Buffer

BACKGROUND: Reteplase is a nonglycosylated derivative of recombinant tissue plasminogen activator, a thrombolytic agent, which can be easily expressed in Escherichia coli. However, overexpression of reteplase in E. coli usually leads to accumulation of insoluble and inactive aggregates and inclusion...

Descripción completa

Detalles Bibliográficos
Autores principales: Fazeli, Behnaz, Akbari, Vajihe, Barkhordari, Asgar, Mir Mohammad Sadeghi, Hamid
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Wolters Kluwer - Medknow 2019
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6839271/
https://www.ncbi.nlm.nih.gov/pubmed/31737582
http://dx.doi.org/10.4103/abr.abr_212_18

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6839271/
https://www.ncbi.nlm.nih.gov/pubmed/31737582
http://dx.doi.org/10.4103/abr.abr_212_18

Ejemplares similares