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Improvement of Soluble Production of Reteplase in Escherichia coli by Optimization of Chemical Chaperones in Lysis Buffer

BACKGROUND: Reteplase is a nonglycosylated derivative of recombinant tissue plasminogen activator, a thrombolytic agent, which can be easily expressed in Escherichia coli. However, overexpression of reteplase in E. coli usually leads to accumulation of insoluble and inactive aggregates and inclusion...

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Detalles Bibliográficos
Autores principales:	Fazeli, Behnaz, Akbari, Vajihe, Barkhordari, Asgar, Mir Mohammad Sadeghi, Hamid
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Wolters Kluwer - Medknow 2019
Materias:	Original Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6839271/ https://www.ncbi.nlm.nih.gov/pubmed/31737582 http://dx.doi.org/10.4103/abr.abr_212_18

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