Multi-Layer Controls of Cas9 Activity Coupled With ATP Synthase Over-Expression for Efficient Genome Editing in Streptomyces

Efficient genome editing is a prerequisite of genetic engineering in synthetic biology, which has been recently achieved by the powerful CRISPR/Cas9 system. However, the toxicity of Cas9, due to its abundant intracellular expression, has impeded its extensive applications. Here we constructed a gene...

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Detalles Bibliográficos
Autores principales: Wang, Kai, Zhao, Qing-Wei, Liu, Yi-Fan, Sun, Chen-Fan, Chen, Xin-Ai, Burchmore, Richard, Burgess, Karl, Li, Yong-Quan, Mao, Xu-Ming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Bioengineering and Biotechnology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6839703/
https://www.ncbi.nlm.nih.gov/pubmed/31737622
http://dx.doi.org/10.3389/fbioe.2019.00304

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6839703/
https://www.ncbi.nlm.nih.gov/pubmed/31737622
http://dx.doi.org/10.3389/fbioe.2019.00304

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