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Properties and efficient scrap-and-build repairing of mechanically sheared 3’ DNA ends

Repairing of DNA termini is a crucial step in a variety of DNA handling techniques. In this study, we investigated mechanically-sheared DNA 3’-ends (MSD3Es) to establish an efficient repair method. As opposed to the canonical view of DNA terminus generated by sonication, we showed that approximately...

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Detalles Bibliográficos
Autores principales: Ohtsubo, Yoshiyuki, Sakai, Keiichiro, Nagata, Yuji, Tsuda, Masataka
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6841706/
https://www.ncbi.nlm.nih.gov/pubmed/31728420
http://dx.doi.org/10.1038/s42003-019-0660-7