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Quantifying cellulose accessibility during enzyme-mediated deconstruction using 2 fluorescence-tagged carbohydrate-binding modules

Two fluorescence-tagged carbohydrate-binding modules (CBMs), which specifically bind to crystalline (CBM2a-RRedX) and paracrystalline (CBM17-FITC) cellulose, were used to differentiate the supramolecular cellulose structures in bleached softwood Kraft fibers during enzyme-mediated hydrolysis. Differ...

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Detalles Bibliográficos
Autores principales: Novy, Vera, Aïssa, Kevin, Nielsen, Fredrik, Straus, Suzana K., Ciesielski, Peter, Hunt, Christopher G., Saddler, Jack
Formato: Online Artículo Texto
Lenguaje:English
Publicado: National Academy of Sciences 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6842628/
https://www.ncbi.nlm.nih.gov/pubmed/31636211
http://dx.doi.org/10.1073/pnas.1912354116