One-step efficient generation of dual-function conditional knockout and geno-tagging alleles in zebrafish

CRISPR/Cas systems are widely used to knock out genes by inducing indel mutations, which are prone to genetic compensation. Complex genome modifications such as knockin (KI) might bypass compensation, though difficult to practice due to low efficiency. Moreover, no ‘two-in-one’ KI strategy combining...

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Detalles Bibliográficos
Autores principales: Li, Wenyuan, Zhang, Yage, Han, Bingzhou, Li, Lianyan, Li, Muhang, Lu, Xiaochan, Chen, Cheng, Lu, Mengjia, Zhang, Yujie, Jia, Xuefeng, Zhu, Zuoyan, Tong, Xiangjun, Zhang, Bo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2019
Materias:
Genetics and Genomics
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6845224/
https://www.ncbi.nlm.nih.gov/pubmed/31663848
http://dx.doi.org/10.7554/eLife.48081

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6845224/
https://www.ncbi.nlm.nih.gov/pubmed/31663848
http://dx.doi.org/10.7554/eLife.48081

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