Modifying a covarying protein–DNA interaction changes substrate preference of a site-specific endonuclease

Identifying and validating intermolecular covariation between proteins and their DNA-binding sites can provide insights into mechanisms that regulate selectivity and starting points for engineering new specificity. LAGLIDADG homing endonucleases (meganucleases) can be engineered to bind non-native t...

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Detalles Bibliográficos
Autores principales: Laforet, Marc, McMurrough, Thomas A, Vu, Michael, Brown, Christopher M, Zhang, Kun, Junop, Murray S, Gloor, Gregory B, Edgell, David R
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2019
Materias:
Nucleic Acid Enzymes
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6847045/
https://www.ncbi.nlm.nih.gov/pubmed/31602462
http://dx.doi.org/10.1093/nar/gkz866

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6847045/
https://www.ncbi.nlm.nih.gov/pubmed/31602462
http://dx.doi.org/10.1093/nar/gkz866

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