Introducing gene deletions by mouse zygote electroporation of Cas12a/Cpf1

CRISPR-associated (Cas) nucleases are established tools for engineering of animal genomes. These programmable RNA-guided nucleases have been introduced into zygotes using expression vectors, mRNA, or directly as ribonucleoprotein (RNP) complexes by different delivery methods. Whereas microinjection...

Descripción completa

Detalles Bibliográficos
Autores principales: Dumeau, Charles-Etienne, Monfort, Asun, Kissling, Lucas, Swarts, Daan C., Jinek, Martin, Wutz, Anton
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing 2019
Materias:
Original Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6848045/
https://www.ncbi.nlm.nih.gov/pubmed/31482512
http://dx.doi.org/10.1007/s11248-019-00168-9

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6848045/
https://www.ncbi.nlm.nih.gov/pubmed/31482512
http://dx.doi.org/10.1007/s11248-019-00168-9

Ejemplares similares