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Introducing gene deletions by mouse zygote electroporation of Cas12a/Cpf1

CRISPR-associated (Cas) nucleases are established tools for engineering of animal genomes. These programmable RNA-guided nucleases have been introduced into zygotes using expression vectors, mRNA, or directly as ribonucleoprotein (RNP) complexes by different delivery methods. Whereas microinjection...

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Detalles Bibliográficos
Autores principales:	Dumeau, Charles-Etienne, Monfort, Asun, Kissling, Lucas, Swarts, Daan C., Jinek, Martin, Wutz, Anton
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Springer International Publishing 2019
Materias:	Original Paper
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6848045/ https://www.ncbi.nlm.nih.gov/pubmed/31482512 http://dx.doi.org/10.1007/s11248-019-00168-9

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