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Label‐Free Fluorescent Kinase and Phosphatase Enzyme Assays with Supramolecular Host‐Dye Pairs

The combination of the macrocyclic hosts p‐sulfonatocalix[4]arene and cucurbit[7]uril with the fluorescent dyes lucigenin and berberine affords two label‐free enzyme assays for the detection of kinase and phosphatase activity by fluorescence monitoring. In contrast to established assays, no substrat...

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Detalles Bibliográficos
Autores principales: Liu, Yan‐Cen, Peng, Shu, Angelova, Lora, Nau, Werner M., Hennig, Andreas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6848908/
https://www.ncbi.nlm.nih.gov/pubmed/31741820
http://dx.doi.org/10.1002/open.201900299