Confident phylogenetic identification of uncultured prokaryotes through long read amplicon sequencing of the 16S‐ITS‐23S rRNA operon

Amplicon sequencing of the 16S rRNA gene is the predominant method to quantify microbial compositions and to discover novel lineages. However, traditional short amplicons often do not contain enough information to confidently resolve their phylogeny. Here we present a cost‐effective protocol that am...

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Detalles Bibliográficos
Autores principales: Martijn, Joran, Lind, Anders E., Schön, Max E., Spiertz, Ian, Juzokaite, Lina, Bunikis, Ignas, Pettersson, Olga V., Ettema, Thijs J. G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley & Sons, Inc. 2019
Materias:
Research Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6849856/
https://www.ncbi.nlm.nih.gov/pubmed/31012228
http://dx.doi.org/10.1111/1462-2920.14636

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6849856/
https://www.ncbi.nlm.nih.gov/pubmed/31012228
http://dx.doi.org/10.1111/1462-2920.14636

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