Exploring structural dynamics of a membrane protein by combining bioorthogonal chemistry and cysteine mutagenesis

The functional mechanisms of membrane proteins are extensively investigated with cysteine mutagenesis. To complement cysteine-based approaches, we engineered a membrane protein with thiol-independent crosslinkable groups using azidohomoalanine (AHA), a non-canonical methionine analogue containing an...

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Detalles Bibliográficos
Autores principales: Gupta, Kanchan, Toombes, Gilman ES, Swartz, Kenton J
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2019
Materias:
Biochemistry and Chemical Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6850778/
https://www.ncbi.nlm.nih.gov/pubmed/31714877
http://dx.doi.org/10.7554/eLife.50776

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6850778/
https://www.ncbi.nlm.nih.gov/pubmed/31714877
http://dx.doi.org/10.7554/eLife.50776

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