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Time-lapse image analysis for whole colony growth curves and daily distribution of the cell number per colony during the expansion of mesenchymal stem cells

Mesenchymal stem cells from the synovium (synovial MSCs) are attractive for cartilage and meniscus regeneration therapy. We developed a software program that can distinguish individual colonies and automatically count the cell number per colony using time-lapse images. In this study, we investigated...

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Detalles Bibliográficos
Autores principales: Mizuno, Mitsuru, Katano, Hisako, Shimozaki, Yuri, Sanami, Sho, Ozeki, Nobutake, Koga, Hideyuki, Sekiya, Ichiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6856116/
https://www.ncbi.nlm.nih.gov/pubmed/31728017
http://dx.doi.org/10.1038/s41598-019-53383-z