Optimization of culture conditions for the expression of three different insoluble proteins in Escherichia coli

Recombinant protein expression for structural and therapeutic applications requires the use of systems with high expression yields. Escherichia coli is considered the workhorse for this purpose, given its fast growth rate and feasible manipulation. However, bacterial inclusion body formation remains...

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Detalles Bibliográficos
Autores principales: Gutiérrez-González, Matías, Farías, Camila, Tello, Samantha, Pérez-Etcheverry, Diana, Romero, Alfonso, Zúñiga, Roberto, Ribeiro, Carolina H., Lorenzo-Ferreiro, Carmen, Molina, María Carmen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6856375/
https://www.ncbi.nlm.nih.gov/pubmed/31727948
http://dx.doi.org/10.1038/s41598-019-53200-7

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6856375/
https://www.ncbi.nlm.nih.gov/pubmed/31727948
http://dx.doi.org/10.1038/s41598-019-53200-7

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