An efficient protein production system via gene amplification on a human artificial chromosome and the chromosome transfer to CHO cells

Gene amplification methods play a crucial role in establishment of cells that produce high levels of recombinant protein. However, the stability of such cell lines and the level of recombinant protein produced continue to be suboptimal. Here, we used a combination of a human artificial chromosome (H...

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Detalles Bibliográficos
Autores principales: Ohira, Takahito, Miyauchi, Koichi, Uno, Narumi, Shimizu, Noriaki, Kazuki, Yasuhiro, Oshimura, Mitsuo, Kugoh, Hiroyuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6861226/
https://www.ncbi.nlm.nih.gov/pubmed/31740706
http://dx.doi.org/10.1038/s41598-019-53116-2

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6861226/
https://www.ncbi.nlm.nih.gov/pubmed/31740706
http://dx.doi.org/10.1038/s41598-019-53116-2

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