Novel gRNA design pipeline to develop broad-spectrum CRISPR/Cas9 gRNAs for safe targeting of the HIV-1 quasispecies in patients

The CRISPR/Cas9 system has been proposed as a cure strategy for HIV. However, few published guide RNAs (gRNAs) are predicted to cleave the majority of HIV-1 viral quasispecies (vQS) observed within and among patients. We report the design of a novel pipeline to identify gRNAs that target HIV across...

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Detalles Bibliográficos
Autores principales: Sullivan, Neil T., Dampier, Will, Chung, Cheng-Han, Allen, Alexander G., Atkins, Andrew, Pirrone, Vanessa, Homan, Greg, Passic, Shendra, Williams, Jean, Zhong, Wen, Kercher, Katherine, Desimone, Mathew, Li, Luna, C. Antell, Gregory, Mell, Joshua Chang, Ehrlich, Garth D., Szep, Zsofia, Jacobson, Jeffrey M., Nonnemacher, Michael R., Wigdahl, Brian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6864089/
https://www.ncbi.nlm.nih.gov/pubmed/31745112
http://dx.doi.org/10.1038/s41598-019-52353-9

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6864089/
https://www.ncbi.nlm.nih.gov/pubmed/31745112
http://dx.doi.org/10.1038/s41598-019-52353-9

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