Effective CRISPR interference of an endogenous gene via a single transgene in mice

Drawbacks of conditional gene deletion in mice include the need for extensive breeding and, often, a lack of cell type-specificity. CRISPR interference (CRISPRi) is an alternative approach for loss-of-function studies that inhibits expression by guiding a transcriptional repressor to the transcripti...

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Detalles Bibliográficos
Autores principales: MacLeod, Ryan S., Cawley, Keisha M., Gubrij, Igor, Nookaew, Intawat, Onal, Melda, O’Brien, Charles A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6872636/
https://www.ncbi.nlm.nih.gov/pubmed/31754144
http://dx.doi.org/10.1038/s41598-019-53611-6

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6872636/
https://www.ncbi.nlm.nih.gov/pubmed/31754144
http://dx.doi.org/10.1038/s41598-019-53611-6

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