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Transcriptional profiling reveals differentially expressed genes involved in lipid biosynthesis during cacao seed development

Theobroma cacao is a plant of economic value due to the use of its seed lipid for chocolate, confectionery, and cosmetic industries. The seed lipid contains a stable ratio of saturated and unsaturated fatty acids, which determines its unique melting temperature. However, little is known about the mo...

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Autores principales: Li, Fupeng, Wu, Baoduo, Yan, Lin, Hao, Chaoyun, Qin, Xiaowei, Lai, Jianxiong, Song, Yinghui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6872657/
https://www.ncbi.nlm.nih.gov/pubmed/31754164
http://dx.doi.org/10.1038/s41598-019-53959-9
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author Li, Fupeng
Wu, Baoduo
Yan, Lin
Hao, Chaoyun
Qin, Xiaowei
Lai, Jianxiong
Song, Yinghui
author_facet Li, Fupeng
Wu, Baoduo
Yan, Lin
Hao, Chaoyun
Qin, Xiaowei
Lai, Jianxiong
Song, Yinghui
author_sort Li, Fupeng
collection PubMed
description Theobroma cacao is a plant of economic value due to the use of its seed lipid for chocolate, confectionery, and cosmetic industries. The seed lipid contains a stable ratio of saturated and unsaturated fatty acids, which determines its unique melting temperature. However, little is known about the molecular mechanism determining the fatty acid ratio and lipid content in cacao. To gain insight into the unique properties of lipid synthesis in cacao, biochemical and transcriptomic approaches were used to compare the lipid accumulation between high and low lipid content cacao accessions. Lipid accumulation rates and lipid content were different between the two accessions. Moreover, differentially expressed genes were detected between high and low lipid content cacao accessions. The data allowed the identification of distinct candidate genes and furthered our understanding of lipid accumulation, potentially explaining the differences in lipid content between various cacao accessions. The results might be used to develop molecular tools and engineer alternative pathways for cacao breeding with improved lipid production potentials.
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spelling pubmed-68726572019-12-04 Transcriptional profiling reveals differentially expressed genes involved in lipid biosynthesis during cacao seed development Li, Fupeng Wu, Baoduo Yan, Lin Hao, Chaoyun Qin, Xiaowei Lai, Jianxiong Song, Yinghui Sci Rep Article Theobroma cacao is a plant of economic value due to the use of its seed lipid for chocolate, confectionery, and cosmetic industries. The seed lipid contains a stable ratio of saturated and unsaturated fatty acids, which determines its unique melting temperature. However, little is known about the molecular mechanism determining the fatty acid ratio and lipid content in cacao. To gain insight into the unique properties of lipid synthesis in cacao, biochemical and transcriptomic approaches were used to compare the lipid accumulation between high and low lipid content cacao accessions. Lipid accumulation rates and lipid content were different between the two accessions. Moreover, differentially expressed genes were detected between high and low lipid content cacao accessions. The data allowed the identification of distinct candidate genes and furthered our understanding of lipid accumulation, potentially explaining the differences in lipid content between various cacao accessions. The results might be used to develop molecular tools and engineer alternative pathways for cacao breeding with improved lipid production potentials. Nature Publishing Group UK 2019-11-21 /pmc/articles/PMC6872657/ /pubmed/31754164 http://dx.doi.org/10.1038/s41598-019-53959-9 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Li, Fupeng
Wu, Baoduo
Yan, Lin
Hao, Chaoyun
Qin, Xiaowei
Lai, Jianxiong
Song, Yinghui
Transcriptional profiling reveals differentially expressed genes involved in lipid biosynthesis during cacao seed development
title Transcriptional profiling reveals differentially expressed genes involved in lipid biosynthesis during cacao seed development
title_full Transcriptional profiling reveals differentially expressed genes involved in lipid biosynthesis during cacao seed development
title_fullStr Transcriptional profiling reveals differentially expressed genes involved in lipid biosynthesis during cacao seed development
title_full_unstemmed Transcriptional profiling reveals differentially expressed genes involved in lipid biosynthesis during cacao seed development
title_short Transcriptional profiling reveals differentially expressed genes involved in lipid biosynthesis during cacao seed development
title_sort transcriptional profiling reveals differentially expressed genes involved in lipid biosynthesis during cacao seed development
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6872657/
https://www.ncbi.nlm.nih.gov/pubmed/31754164
http://dx.doi.org/10.1038/s41598-019-53959-9
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